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2013 Fiscal Year Final Research Report

Development of a novel cancer vaccine using chemokine CCL21 and oncolytic adenovirus

Research Project

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Project/Area Number 23591973
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionHyogo Medical University

Principal Investigator

YAMANO Tomoki  兵庫医科大学, 医学部, 講師 (00599318)

Co-Investigator(Kenkyū-buntansha) KUBO Shuji  兵庫医科大学, 医学部, 准教授 (10441320)
TOMITA Naohiro  兵庫医科大学, 医学部, 教授 (00252643)
OHYAMA Hideki  兵庫医科大学, 医学部, 准教授 (90280685)
Project Period (FY) 2011 – 2013
Keywords癌ワクチン / 腫瘍溶解ウイルス
Research Abstract

We considered cancer vaccine using cancer cells infected with oncolytic virus as cancer antigen. At first, we assessed the duration of oncolytic virus infection with 104 mouse rectal cancer cells, CT26. The infection time of 24h, not 10h of oncolytic virus with MOI1000 was sufficient for complete inhibition of tumor growth when 104 of untreated CT26 cells were subsequently inoculated. 50% of mice vaccinated by CT26 cells infected with MOI1000 of oncolytic virus rejected subsequently inoculated CT26 cells, although 25% of mice without vaccination rejected subsequently inoculated CT26 cells. When 105 of CT26 cells were used for vaccination, 31% of mice vaccinated by CT26 cells infected with MOI1000 of oncolytic virus or 17% of mice vaccinated by CCL21 protein rejected subsequently inoculated CT26, respectively. All the mice without vaccination bore tumors. These results indicate CT26 cells infected with oncolytic virus are immunogenic to induce immune response to against CT26 cells.

  • Research Products

    (5 results)

All 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Oncolytic virotherapy for osteosarcoma using midkine promoter-regulated adenoviruses2014

    • Author(s)
      Takagi-Kimura Misato, Yamano Tomoki, Tagawa Masatoshi, Kubo Shuji
    • Journal Title

      Cancer gene therapy

      Volume: 21(3) Pages: 126-32

    • Peer Reviewed
  • [Journal Article] Enhanced antitumor efficacy of fiber-modified, midkine promoter-regulated oncolytic adenovirus in human malignant mesothelioma2013

    • Author(s)
      Takagi-Kimura Misato, Yamano Tomoki, Tamamoto Atsuko, Okamura Nobutaka, Okamura Haruki, Hashimoto-Tamaoki Tomoko, Tagawa Masatoshi, Kasahara Noriyuki, Kubo Shuji
    • Journal Title

      Cancer science

      Volume: 104(11) Pages: 1433-9

    • Peer Reviewed
  • [Presentation] ケモカインCCL21と腫瘍溶解アデノウイルスを用いた新しい癌ワクチン療法の開発2014

    • Author(s)
      山野智基,久保秀司,矢野綾,松原長秀,山本英幸,岡村春樹,冨田尚裕
    • Organizer
      日本癌治療学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-08-29
  • [Presentation] 二重制御型アデノウイルスを用いた骨肉腫に対する腫瘍溶解療法の開発2013

    • Author(s)
      久保秀司、木村美智、玉本敦子、山野智基、玉置知子、笠原典之、田川雅敏
    • Organizer
      (一般)日本人類遺伝学会第58回大会
    • Place of Presentation
      仙台
    • Year and Date
      20131100
  • [Presentation] Dual targeted adenovirus for oncolytic virotherapy in experimental human osteosarcoma.(General Lecture)2013

    • Author(s)
      Kubo Shuji, Takagi-Kimura Misato, Yamano Tomoki, Yoshikawa Yoshie,Emi Mitsuru, Tagawa Masatoshi, Kasahara Noriyuki, Hashimoto-Tamaoki Tomoko
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      Yokohama
    • Year and Date
      20131000

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Published: 2015-07-16  

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