A clinical and basic research for developing human small hepatocyte bank using super-cooling program and generating mass hybrid mouse.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591993
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Sapporo Medical University
• Principal Investigator: MIZUGUCHI TORU 札幌医科大学, 医学部, 准教授 (30347174)
• Co-Investigator(Kenkyū-buntansha): HIRATA Koichi 札幌医科大学, 医学部, 教授 (50136959) ; MITAKA Toshihiro 札幌医科大学, 医学部, 教授 (50231618) ; NAKAMURA Yukio 札幌医科大学, 医学部, 研究員 (50516648) ; KAWAMOTO Masaki 札幌医科大学, 医学部, 助教 (70404605) ; MEGURO Makoto 札幌医科大学, 医学部, 助教 (50448601)
• Project Period (FY): 2011 – 2013
• Keywords: 肝臓外科学

Research Abstract

We investigated that basic research for applying clinical liver regenerative therapy and clinical research for securing donor cell procurement. In the basic research, we developed a rat model of liver regenerative therapy by hepatocyte transplantation. In this model, we revealed a cell physiology of the transplanted cells which generated bile duct linage cells. In addition, we succeded to increase survival rate in NASH liver cirrhotic model. In the clinical research, we reported a benefit and default of donor cell procurement. We also revealed that a clinical value of BTR, ATIII, and GSA. We classified various liver functional indicators using clustering analysis. Furthermore, in technical reports, we developed a surgical devise for conducting safety right liver hepatectomy (J Am Col Surg : in press).

Research Products

• [Journal Article] Propensity score analysis demonstrated the prognostic advantage of anatomical liver resection in hepatocellular carcinoma (2014)
  • Author(s): Ishii S, Mizuguchi T, Kawamoto M, Meguro M, Ota S, Nishidate T, Okita K, Kimura Y, Hui TT, Hirata K.
  • Journal Title: World J Gastroenterol Volume: 20 Pages: 3335-42
  • DOI: 10.3748/wjg.v20.i12.3335
  • Peer Reviewed
• [Journal Article] Highest intraoperative lactate level could predict postoperative infectious complications after hepatectomy, reflecting the Pringle maneuver especially in chronic liver disease (2014)
  • Author(s): Meguro M, Mizuguchi T, Kawamoto M, Nishidate T, Ishii M, Tatsumi H, Kimura Y, Furuhata T, Hirata K.
  • Journal Title: J Hepatobiliary Pancreat Sci.
  • DOI: 10.1002/jhbp.87
  • Peer Reviewed
• [Journal Article] Preoperative liver function assessments to estimate the prognosis and safety of liver resections (2014)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Hui TT, Hirata K.
  • Journal Title: Surg Today Volume: 44 Pages: 1-10
  • DOI: 10.1007/s00595-013-0534-4
  • Peer Reviewed
• [Journal Article] New technique of extracorponeal hepatic inflow control for pure laparoscopic liver resection (2013)
  • Author(s): Mizuguchi T, Kawamoto M, Nakamura Y, Meguro M, Harada K, OtaS , Hui TT, and Hirata K
  • Journal Title: Surg Laparosc Endosc Percutan Tech Volume: (in press)
  • Peer Reviewed
• [Journal Article] Prediction of postoperative liver failure and evaluation of modified criteria for liver resection with computed volume analysis (2013)
  • Author(s): Harada K, Mizuguchi T, Kawamoto M, Meguro M, Ota S, Sasaki S, Miyanishi K, Hatakenaka M, Shinomura Y, Kato J, Hirata K.
  • Journal Title: Hepatogastroenterology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Left Lateral Sectionectomy Performed Under Minimal Open Access after the Completion of Hand-Assisted Laparoscopic Mobilization (2013)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Ota S, Ishii S, Okita K, Kimura Y, Furuhata T, Hirata K.
  • Journal Title: J Liver Volume: 2 Pages: 5
  • DOI: 10.4172/2167-0889.1000141
  • Peer Reviewed
• [Journal Article] Asialoglycoprotein receptor scintigraphy with 99mTc-galactosyl human serum albumin (99mTc-GSA) as an early predictor of survival in acute liver failure (2013)
  • Author(s): Tatsumi H, Masuda Y, Imaizumi H, Yoshida S, Goto K, Yama N, Mizuguchi T, Hirata K.
  • Journal Title: Anaesth Intensive Care Volume: 41 Pages: 523-8
  • URL: http://www.aaic.net.au/Document/?D=20130021
  • Peer Reviewed
• [Journal Article] Differentiation capacity of hepatic stem/progenitor cells isolated from D-galactOsamine-treated rat livers (2013)
  • Author(s): Ichinohe N, Tanimizu N, Ooe H, Nakamura Y, Mizuguchi T, Kon J, Hirata K, Mitaka T.
  • Journal Title: Hepatology Volume: 57 Pages: 1192-202
  • DOI: 10.1002/hep.26084
  • Peer Reviewed
• [Journal Article] Hepatic biliary epithelial cells acquire epithelial integrity but lose plasticity to differentiate into hepatocytes in vitro during development (2013)
  • Author(s): Tanimizu N, Nakamura Y, Ichinohe N, Mizuguchi T, Hirata K, Mitaka T.
  • Journal Title: J Cell Sci. Volume: 126 Pages: 5239-46
  • DOI: 10.1242/jcs.133082
  • Peer Reviewed
• [Journal Article] Prognosis and Predictors of Surgical Complications in Hepatocellular Carcinoma Patients With or Without Cirrhosis after Hepatectomy (2013)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Nakamura Y, Ota S, Hui TT, Hirata K.
  • Journal Title: World J Surg Volume: 37 Pages: 1379-87
  • DOI: 10.1007/s00268-013-1989-6
  • Peer Reviewed
• [Journal Article] Continuous monitoring of central venous oxygen saturation predicts postoperative liver dysfunction after liver resection (2013)
  • Author(s): Meguro M, Mizuguchi T, Kawamoto M, Nakamura Y, Ota S, Kukita K, Ishii M, Tatsumi H, Hirata K.
  • Journal Title: Surgery Volume: 154 Pages: 351-62
  • DOI: 10.1016/j.surg.2013.04.039
  • Peer Reviewed
• [Journal Article] Preoperative hepatocyte transplantation improves the survival of rats with non -alcoholic steatohepatitis-related cirrhosis after partial hepatectomy (2013)
  • Author(s): Nakamura Y, Mizuguchi T, Tanimizu N, Ichinohe N, Ooe H, Kawamoto M, Meguro M, Hirata K, Mitaka T.
  • Journal Title: Cell Transplant
  • DOI: 10.3727/096368913X668649
  • Peer Reviewed
• [Journal Article] Serum antithrombin III level is well correlated with multiple indicators for assessment of liver function and diagnostic accuracy for predicting postoperative liver failure in hepatocellular carcinoma patients (2012)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Son S, Nakamura Y, Harada K, Shibata T, Ota S, Hirata K.
  • Journal Title: Hepatogastroenterology Volume: 59 Pages: 551-7
  • DOI: 10.5754/hge10052
  • Peer Reviewed
• [Journal Article] Low dose steroid pretreatment ameliorates transient impairment of the liver regeneration due to radiofrequency ablation (2012)
  • Author(s): Shibata T, Mizuguchi T, Nakamura Y, Kawamoto M, Meguro M, Ota S, Hirata K, Ooe H, Mitaka T.
  • Journal Title: World J Gastroenterol Volume: 18 Pages: 905-14
  • DOI: 10.3748/wjg.v18.i9.905
  • Peer Reviewed
• [Journal Article] Area among the hepatic and heart curves of 99mTc-galactocyl-human serum albumin scintigraphy represents liver function and disease progression for preoperative evaluation in hepatocellular carcinoma patients (2012)
  • Author(s): Harada K, Mizuguchi T, Katagiri Y, Kawamoto M, Nakamura Y, Meguro M, Ota S, Sasaki S, Miyanishi K, Sonoda T, Shinomura Y, Hirata K.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 19 Pages: 667-73
  • DOI: 10.1007/s00534-011-0486-2
  • Peer Reviewed
• [Journal Article] The molecular pathogenesis and clinical implications of hepatocellular carcinoma (2011)
  • Author(s): Meguro M, Mizuguchi T, Kawamoto M, Hirata K.
  • Journal Title: Int J Hepatol Volume: 2011 Pages: 818672
  • DOI: 10.4061/2011/818672
  • Peer Reviewed
• [Journal Article] Laparoscopic hepatectomy : a systematic review, meta-analysis and power analysis (2011)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Shibata T, Nakamura Y, Kimura Y, Furuhata T, Sonoda T, Hirata K.
  • Journal Title: Surg Today Volume: 41 Pages: 39-47
  • DOI: 10.1007/s00595-010-4337-6
  • Peer Reviewed
• [Journal Article] Prognostic impact of preoperative branched-chain amino acids to the tyrosine ratio level in hepatocellular carcinoma patients after initial hepatectomy (2011)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Nakamura Y, Harada K, Kukita K, Hirata K.
  • Journal Title: J Gastroint Surg Volume: 15 Pages: 1433-9
  • DOI: 10.1007/s11605-011-1566-y
  • Peer Reviewed
• [Journal Article] Cluster analysis of liver functional indicators and preoperative low BTR indicate high risk of early recurrence in analysis of 165 HCC patients after initial hepatectomy (2011)
  • Author(s): Nakamura Y, Mizuguchi T, Kawamoto M, Meguro M, Harada K, Ota S, Hirata K.
  • Journal Title: Surgery Volume: 150 Pages: 250-62
  • DOI: 10.1016/j.surg.2011.06.001
  • Peer Reviewed
• [Journal Article] Saline injection method for facilitating the liver hanging maneuver during hepatectomy for a large right liver tumor
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Okita K, Nishidate T, Furuhata T, Hui TT, Hirata K.
  • Journal Title: J Am Col Surg Volume: (in press)
  • Peer Reviewed
• [Journal Article] The impact of aging on morbidity and mortality after liver resection : a systematic review and meta-analysis
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Okita K, Ota S, Ishii S, Ueki T, Kimura Y, Furuhata T, Hirata K.
  • Journal Title: Surg Today Volume: (in press)
  • Peer Reviewed
• [Presentation] Prognostic Impact of Aldehyde Dehydrogenase 1 Expression in Viral- and Alcohol-Unrelated Hepatocellular Carcinoma (2014)
  • Author(s): Mizuguchi T, Kawamoto K, S. Ota, M. Ishii, Meguro M, Okita K, Furuhata T, Nobuoka T, Kimura Y, Hirata K.
  • Organizer: 9th Annual Academic Surgical Congress, Quick shot oral presentation
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 20140204-06
• [Presentation] Continuous monitoring of central venous oxygen saturation predicts postoperative liver dysfunction after liver resection (2013)
  • Author(s): Meguro M, Mizuguch T, Kawamoto M, Nakamura Y, Kukita K, Ota S, Hirata K.
  • Organizer: 8th Annual Academic Surgical Congress, Oral presentation
  • Place of Presentation: New Orleans, LO, USA
  • Year and Date: 20130205-07
• [Presentation] Hepatic Progenitor Cell Transplantation for Improving Survival after Liver Resection of Rat Non-alcoholic Steatocirrhotic Liver Model (2012)
  • Author(s): Nakamura Y, Mizuguchi T, Ohe H, Kawamoto K, Meguro M, Ota S, Mitaka T, Hirata K.
  • Organizer: 7th Annual Academic Surgical Congress, Quick shot oral presentation
  • Place of Presentation: Las Vegas, NV, USA
  • Year and Date: 20120214-16
• [Presentation] Hirata Extracorporeal hepatic inflow control method for pure laparoscopic liver resection (2012)
  • Author(s): Mizuguchi T, Kawamoto K, Meguro M, Nakamura Y, Harada K, Nobuoka T, Kimura Y, Furuhata T.
  • Organizer: 7th Annual Academic Surgical Congress, Quick shot oral presentation
  • Place of Presentation: Las Vegas, NV, USA
  • Year and Date: 20120214-16
• [Book] Role of Branched Chain Amino Acids in Cellular and Organ Damage : The prognostic significance of the preoperative branched chain amino acid to tyrosine ratio. In The branced chain amino acids in health and disease (2014)
  • Author(s): Mizuguchi T, Mitaka T, Hirata K.
  • Publisher: Springer(In press)
• [Patent(Industrial Property Rights)] 医療用結紮テープ保持具 (2011)
  • Inventor(s): 水口徹、平田公一、川本雅樹、目黒誠、岩立力、日置元久
  • Industrial Property Rights Holder: 北海道公立大学法人 札幌医科大学、株式会社 河野製作所
  • Industrial Property Number: 2011-026442
  • Filing Date: 2011-11-15