2013 Fiscal Year Final Research Report
Ischemia/Reperfusion-induced Myocardial Damage: Role of Carbon Dioxide
| Project/Area Number |
23592023
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | Akita University |
|---|
Principal Investigator |
YAMAMOTO Hiroshi 秋田大学, 医学(系)研究科(研究院), 准教授 (10270795)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Fumio 秋田大学, 大学院医学系研究科, 教授 (00127474)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 心筋虚血再灌流 / 二酸化炭素 / ナトリウム/水素交換 / ナトリウム/カルシウム交換 |
|---|
| Research Abstract |
We investigated the acid-base characteristic alterations of blood cardioplegia (BCP) during aortic cross-clamping in hearts arrested with BCP and during in vitro simulated ischemia. In 40 patients undergoing cardiac surgery following an aortic cross clamp, hearts were infused with a hypothermic BCP intermittently and lastly with a normothermic BCP prior to aortic cross clamp release. We measured pH, pCO2, [HCO3-], and [Cl-] of the coronary sinus effluent in the final BCP. BCP was assessed under in-vitro gassing at with 95% N2 + 5% CO2 (n = 6), 50% N2 + 50% CO2 (n = 3), or 100% CO2 (n = 6). The coronary sinus effluent, compared with the pre-infused BCP, exhibited significantly lower pH and greater pCO2 with no change in the [HCO3-] level. In vitro, the 100% CO2 gassing groups exhibited a significant increase in [HCO3-] under high pCO2-induced acidification. Under anoxia and CO2 retention during aortic cross-clamping, BCP can be a bicarbonate donor to the myocardium.
|
