The role of Necl-5 in the invasive activity of lung adenocarcinoma

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592060
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kobe University
• Principal Investigator: MANIWA YOSHIMASA 神戸大学, 医学(系)研究科(研究院), 教授 (50362778)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Yoshitake 神戸大学, 大学院保健学研究科, 教授 (50189669)
• Project Period (FY): 2011 – 2013
• Keywords: 原発性肺癌 / 肺腺癌 / 細胞接着分子 / Necl5 / 浸潤能 / 上皮間葉移行 / 分子標的 / バイオマーカー

Research Abstract

In this study,we used a bronchioloalveolar carcinoma (BAC) cell line and fibroblasts to assess the expression of Necl-5 in the development of cancer–stroma communication by using an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) systemthat enables visualization of cellmigration during invasion. The expression of Necl-5 was higher in BAC cells than in fibroblasts. This tendency didn't change even when the BAC cells were mixed with fibroblasts. To assess the role of Necl-5 in the invasive activity of the BAC cells, we knocked down its expression using RNA interference (RNAi). The invasion assay with DL-CGH revealed that inhibition of Necl-5 expression in the BAC cells was associated with suppressed invasiveness. Necl-5 expression in lung cancer cells is crucial for their invasiveness in the cancer–stromal interaction, suggesting that Necl-5 could be a favorable molecular target for the suppression of invasiveness in lung adenocarcinoma.

Research Products

• [Journal Article] Slug increases sensitivity to tubulin-binding agents via the downregulation ofβIII andβIVa-tubulin in lung cancer cells (2013)
  • Author(s): Tamura D, Arao T, Nagai T, Kaneda H, Aomatsu K, Fujita Y, Matsumoto K, De Velasco MA, Kato H, Hayashi H, Yoshida S, Kimura H, Maniwa Y, Nishio W, Sakai Y, Ohbayashi C, Kotani Y, Nishimura Y, Nishio K.
  • Journal Title: Cancer Med Volume: 2 Pages: 144-54
  • Peer Reviewed
• [Journal Article] The role of Necl-5 in the invasive activity of lung adenocarcinoma (2013)
  • Author(s): Tane S, Maniwa Y, Hokka D, Tauchi S, Nishio W, Okita Y, Yoshimura M
  • Journal Title: Exp Mol Pathol Volume: 94(2) Pages: 330-5
  • Peer Reviewed
• [Journal Article] Immunohistochemical studies of pulmonary large cell neuroendocrine carcinoma : A possible association between staining patterns with neuroendocrine markers and tumor response to chemotherapy (2013)
  • Author(s): Tanaka Y, Ogawa H, Uchino K, Ohbayashi C, Maniwa Y, Nishio W, Nakao A, Yoshimura M
  • Journal Title: J Thorac Cardiovasc Surg Volume: 145 Pages: 839-846
  • Peer Reviewed
• [Journal Article] Psf3 is a prognostic biomarker in lung adenocarcinoma (2013)
  • Author(s): Hokka D, Maniwa Y, Tane S, Nishio W, Yoshimura M, Okita Y, Ohbayashi C, Sakai Y, Chen X, Hayashi Y
  • Journal Title: Lung Cancer Volume: 79 Pages: 77-82
  • Peer Reviewed
• [Journal Article] Expression of Nectin 3: novel prognostic marker of lung adenocarcinoma (2012)
  • Author(s): Maniwa Y, Nishio W, Okita Y, Yoshimura
  • Journal Title: Thoracic Cancer Volume: 3 Pages: 175-181
  • Peer Reviewed
• [Presentation] 肺腺癌浸潤におけるNecl-5の働きについての検討 (2013)
  • Author(s): 田根慎也、真庭謙昌
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2013-04-11