New role of stem cells in the GFP-rat lung transplantation model

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Nagasaki University
• Principal Investigator: YAMASAKI Naoya 長崎大学, 医歯(薬)学総合研究科, 准教授 (70404217)
• Co-Investigator(Kenkyū-buntansha): TSUCHIYA Tomoshi 長崎大学, 大学院医歯薬学総合研究科, 講師 (30437884) ; NAGAYASU Takeshi 長崎大学, 大学院医歯薬学総合研究科, 教授 (80284686) ; MATSUMTO Keitaro 長崎大学, 大学病院, 助教 (80404268) ; MIYAZAKI Takuro 長崎大学, 大学病院, 助教 (00584749)
• Project Period (FY): 2011 – 2013
• Keywords: 肺移植 / 脂肪幹細胞 / 免疫寛容 / 拒絶 / 量子ドット

Research Abstract

We established rat lung transplantation model. 2 weeks before the lung transplantation, we harvested abdominal adipose tissue from recipients, extracted adipose tissue stromal cells (ADSCs) and cultivated. We divided groups into AD (Intravenous administration of cultivated ADSCs), F (Tacrolimus administration after lung transplantation), D (ADSCs and tacrolimus intravenously after lung transplantation), and C (control). In the results, the rejection grade of AF group was lower than that of F group. The PCNA labeling index was low in AF group. In AF group, serum HGF and IL-10 were high and serum IL-2 was low, suggesting rejection of AF was low serologically. Further, Dot blot was used instead of GFP and we could observe Dot blot stained adipose stem cell in the lung in AD and D groups.