Mechanism of glioma invasion and its inhibition

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: University of Fukui
• Principal Investigator: KITAI Ryuhei 福井大学, 医学部, 准教授 (80251990)
• Project Period (FY): 2011 – 2013
• Keywords: Glioma / Brain tumor / Invasion / Detection

Research Abstract

The motility of cultured glioma cells were estimated by video monitor. The glioma cell line A172 was the most moving cells in other cells such as U87, T98G and primary glioma cell culture. The cells stimulated by low Oxygen chamber and low pH showed initial cell halt and following high motility. The inhibition of these motility was blocked by interferon beta. As for the detection of invasion front, recently 5ALA photodynamic diagnosis was introduced in glioma surgery. Using cultured cells, the fluorescent intensity and cell density were determined. The detection limit by the operator's naked eyes was reported.

Research Products

• [Journal Article] Tumor cell density required for macroscopic observation of 5-ALA-induced fluorescence of protoporphyrin-IX: Measurement in cultured glioma cells and clinical cases (2014)
  • Author(s): Kitai R, Takeuchi H, N Miyoshi, et al
  • Journal Title: No Shinkei Geka Volume: (in press)
  • Peer Reviewed
• [Journal Article] A Large Intramedullary Neurofibroma in the Thoracic Spinal Cord: Case Report (2014)
  • Author(s): Arishima H, Kitai R, Kodera T, Yamada S, Kikuta KI
  • Journal Title: Neurol Med Chir
• [Journal Article] The adaptation of a neuroendoscopic sheath to a Leksell stereotactic frame to make it suitable for approaching small ventricles: a technical note (2014)
  • Author(s): Kitai R, Nakano K, Hashimoto N, Arishima H, Yamauchi T, Higashino Y, Takeuchi H, Kikuta K. J Neurol Surg A
  • Journal Title: J Neurol Surg A Cent Eur Neurosurg Volume: 75 Pages: 151-4
  • Peer Reviewed
• [Journal Article] Frontal meningioma incidentally detected in the routine preoperative assessment of tongue cancer: a case report and literature review (2013)
  • Author(s): Sano K, Kitai R, Yoshimura H, Ohba S
  • Journal Title: J Oral Maxillofac Surg. Volume: 2195 Pages: e1-6
  • Peer Reviewed
• [Journal Article] Intraoperative computed tomography for cervicomedullary decompression of foramen magnum stenosis in achondroplasia: two case reports (2013)
  • Author(s): Arishima H, Tsunetoshi K, Kodera T, Kitai R, Takeuchi H, Kikuta K
  • Journal Title: Neurol Med Chir Volume: 53 Pages: 902-6
  • Peer Reviewed
• [Journal Article] Coincident choroid plexus carcinoma and adrenocortical tumor in an infant (2013)
  • Author(s): Yoshida K, Sato K, Kitai R, Hashimoto N, Kubota T, Kikuta K
  • Journal Title: Brain Tumor Pathol Volume: 30(2) Pages: 104-8
  • Peer Reviewed
• [Journal Article] Measurement and cellular sources of the soluble interleukin-2 receptor in primary central nervous system lymphoma (2013)
  • Author(s): Kitai R, Sasaki H, Matsuda K, Tsunetoshi K, Yamauchi T, Neishi H, Matsumura K, Tsunoda A, Takeuchi H, Sato K, Kikuta K
  • Journal Title: Brain Tumor Pathol Volume: (1) Pages: 34-9
  • Peer Reviewed
• [Journal Article] Clinicopathological features in the recurrence of oligodendroglioma and diffuse astrocytoma (2012)
  • Author(s): Takeuchi H, Hosoda T, Kitai R, Kodera T, Arishima H, Tsunetoshi K, Neishi H, Yamauchi T, Sato K, Imamura Y, Itoh H, Kubota T, Kikuta K
  • Journal Title: Brain Tumor Pathol Pages: 140-7
  • Peer Reviewed
• [Journal Article] Glioblastoma with oligodendroglial components (2012)
  • Author(s): Takeuchi H, Hosoda T, Kitai R, Kodera T, Arishima H, Tsunetoshi K, Neishi H, Yamauchi T, Sato K, Imamura Y, Itoh H, Kubota T, Kikuta K
  • Journal Title: Brain Tumor Pathol Volume: (3) Pages: 154-9
  • Peer Reviewed
• [Journal Article] Lymphomatosis cerebri : clinical characteristics, neuroimaging, and pathological findings (2012)
  • Author(s): Kitai R, Hashimoto N, Yamate K, Ikawa M, Yoneda M, Nakajima T, Arishima H, Takeuchi H, Sato K, Kikuta K
  • Journal Title: Brain Tumor Pathol. Volume: (1) Pages: 47-53
  • Peer Reviewed
• [Journal Article] Usefulness of intraoperative computed tomography in surgery for low-grade gliomas : a comparative study between two series without and with intraoperative computed tomography (2011)
  • Author(s): Hosoda T, Takeuchi H, Hashimoto N, Kitai R, Arishima H, Kodera T, Higashino Y, Sato K, Kikuta K
  • Journal Title: Neurol Med Chir Volume: 51(7) Pages: 490-5
  • Peer Reviewed
• [Presentation] High Grade Glioma術中5ALA蛍光診断における限界蛍光強度の定量化細胞培養と臨床検体の対比 (2012)
  • Author(s): 北井隆平
  • Organizer: 日本脳神経外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2012-10-18
• [Presentation] 病理組織からみた膠芽腫の浸潤境界および再発GBMにおける5ALA蛍光手術の限界 (2012)
  • Author(s): 北井隆平
  • Organizer: 脳腫瘍病理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-05-25
• [Book] 病気の分子形態学Central neurocytoma (2011)
  • Total Pages: 163-165
  • Publisher: 学際企画
• [Remarks]
  • URL: http://www.med.u-fukui.ac.jp/NOUGE/