2013 Fiscal Year Final Research Report
Malignant glioma tropism of induced pluripotent stem cell
| Project/Area Number |
23592119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAMBA Hiroki 浜松医科大学, 医学部, 教授 (60198405)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 人工多能性幹細胞 / 悪性グリオーマ / 移動能 |
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| Research Abstract |
In the present study, we tested tumor tropic activity of mouse iPSCs using in vitro Matrigel Invasion assay and in vivo mouse intracranial tumor model. Mouse iPS cells showed a significant tropism to the conditioned media prepared from glioma cell lines and this tropism to the glioma conditioned media was partially blocked by the neutralizing antibodies for four major tumor-associated growth factors. The receptors for those growth factors, measured by reverse transcriptase polymerase chain reaction, were highly up-regulated in the mouse iPS cells compared to the mouse fibroblasts. Mouse iPSCs labeled with 5-bromo-2-deoxyuridine were intracranially implanted in the contralateral hemisphere to the GL261 gliomacell implantation in the allogeneic C57BL/6 mouse. Active migration of iPSCs was observed 7 days after implantation. These findings demonstrated that iPSCs had potent glioma tropism and could be candidates as vehicles in stem cell-based glioma therapy.
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Research Products
(4 results)
