• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Oct-3/4 promotes tumor angiogenesis through VEGF production in glioblastoma

Research Project

  • PDF
Project/Area Number 23592129
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cerebral neurosurgery
Research InstitutionEhime University

Principal Investigator

HISAAKI Takahashi  愛媛大学, 医学(系)研究科(研究院), 講師 (20363228)

Project Period (FY) 2011 – 2013
Keywords膠芽腫 / Oct-3/4 / 血管新生 / VEGF / HIF1 / AKT
Research Abstract

Accumulating evidence shows that the expression level of Oct-3/4, a self-renewal regulator in stem cells, is positively correlated with the progression of various solid tumors. However, little is known regarding the influence of Oct-3/4 in the tumor angiogenesis of glioblastoma. In this study, we subcutaneously transplanted U251/EGFP-Oct-3/4 cells into nude mice to evaluate the roles of Oct-3/4 in the tumor angiogenesis. Both tumor size and the number of large vessels growing in the tumor were markedly increased. In an in vitro model of angiogenesis, conditioned media from U251/EGFP-Oct-3/4 cells significantly accelerated capillary-like tube formation. In U251/EGFP-Oct-3/4 cells, VEGF mRNA was upregulated under the control of HIF1a, and enhanced protein expression and nuclear translocation of HIF1a were observed. Our results demonstrate that Oct-3/4-expressing glioma cells have the ability to adapt to low-oxygen environments of tumor by promoting tumor angiogenesis.

  • Research Products

    (9 results)

All 2014 2013 2012 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Activated microglia in a rat stroke model express NG2 proteoglycan in peri-infarct tissue through the involvement of TGF-β12014

    • Author(s)
      Sugimoto K, Nishioka R, Ikeda A, Mise A, Takahashi H, Yano H, Kumon Y, Ohnishi T, Tanaka J
    • Journal Title

      GLIA

      Volume: 62 Pages: 185-198

    • Peer Reviewed
  • [Journal Article] Expression of MCP-1 and fractalkine on endothelial cells and astrocytes may contribute to the invasion and migration of brain macrophages in ischemic rat brain lesions2013

    • Author(s)
      Tei N, Tanaka J, Sugimoto K, Nishihara T, Nishioka R, Takahashi H, Yano H, Matsumoto S, Ohue S, Watanabe H, Kumon Y, Ohnishi T
    • Journal Title

      J Neurosci Res

      Volume: 91 Pages: 681-693

    • Peer Reviewed
  • [Journal Article] Postnatal interleukin-1βenhances adulthood seizure susceptibility and neuronal cell death after prolonged experimental febrile seizures in infantile rats2013

    • Author(s)
      Fukuda M, Hino H, Suzuki Y, Takahashi H, Morimoto T, Ishii E
    • Journal Title

      Acta Neurologica Belgica

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Oct-3/4 promotes migration and invasion of glioblastoma cells2012

    • Author(s)
      Kobayashi K, Takahashi H, Inoue A, Harada H, Toshimori S, Kobayashi Y, Goto K, Sugimoto K, Yano H, Ohnishi T, Tanaka J
    • Journal Title

      J Cell Biochem

      Volume: 113(2) Pages: 508-517

    • Peer Reviewed
  • [Journal Article] Anticonvulsive effect of paeoniflorin on experimental febrile seizures in immature rats : possible application for febrile seizures in children2012

    • Author(s)
      Hino H, Takahashi H, Suzuki Y, Tanaka J, Ishii E, Fukuda M
    • Journal Title

      PLoS One

      Volume: 7 Pages: e42920

    • Peer Reviewed
  • [Presentation] Oct-3/4 promotes tumor angiogenesis in glioblastoma through VEGF production2014

    • Author(s)
      Takahashi H, Kawabe Y, Iwata S, Sugimoto K, Yano H, Tanaka J
    • Organizer
      日本生理学会
    • Place of Presentation
      鹿児島
    • Year and Date
      20140316-18
  • [Presentation] Oct-3/4 induces CpG demethylation in MGMT promoter to acquire temozolomide resistance in glioblastoma cells2013

    • Author(s)
      Takahashi H, Inoue A, Kobayashi Y, Hosokawa Y, Harada H, Ohnishi T, Sugimoto K, Yano H, Tanaka J
    • Organizer
      日本生理学会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] Oct-3/4 promotes migration and invasion of glioblastoma cells2012

    • Author(s)
      Takahashi H, Kobayashi K, Inoue A, Harada H, Sugimoto K, Yano H, Ohnishi T, Tanaka J
    • Organizer
      日本生理学会
    • Place of Presentation
      松本
    • Year and Date
      20120329-31
  • [Remarks]

    • URL

      http://hgyano.jimdo.com/

URL: 

Published: 2015-07-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi