Evaluation of safety and effectiveness of tissue-adhesive hydrogel for the development of hemostatic agent in neurosurgery

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Osaka City University
• Principal Investigator: KENJI Ohata 大阪市立大学, 医学(系)研究科(研究院), 教授 (70194264)
• Co-Investigator(Kenkyū-buntansha): NAGASAKI Takeshi 大阪市立大学, 大学院工学研究科, 教授 (30237507) ; ISHIBASHI Kenichi 大阪市立大学, 大学院医学研究科, 講師 (50438230)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: フィブリノーゲン / 特定生物由来製品 / ポリアルデヒド / ポリアミン / ハイドロゲル / 架橋反応 / 安全性 / 有効性

Outline of Final Research Achievements

Combination of fibrinogen and trombone has been used for the purpose of hemostats and tissue-adhesions. In this work, novel cell adhesive hydrogels were developed by using naturally occurring polymers, ε-poly-L-lysine and β-1,3-glucan (Aquaβ). Storage elastic modulus (G’) with nEG-PLL and CHO-Aquaβ showed a higher value than that of a conventional hemostatic agent. Since our hydrogels are consisting of naturally occurring polymers, they have little risk of infectious contaminations. When the hydrogels and their starting materials were administrated by a single oral gavage, no obvious acute toxicity was observed not only on blood chemistry, but also in inflammatory parameters. The results showed that hydrogels obtained by the schiff base formation between nEG-PLL and CHO-Aquaβ provides a great potential for safer and effective hemostatic agent.

Free Research Field

脳神経外科