2013 Fiscal Year Final Research Report
Mechanism of heterotopic ossification in human spinal ligament and skin tissues of patient with ossification of spinal ligaments
Project/Area Number |
23592152
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
|
Research Institution | Hirosaki University |
Principal Investigator |
NUMASAWA Takuya 弘前大学, 医学(系)研究科(研究院), 研究員 (80396407)
|
Project Period (FY) |
2011 – 2013
|
Keywords | 幹細胞 / 靱帯 / 皮膚 / 異所性骨化 |
Research Abstract |
We isolated and expanded MSCs from human spinal ligaments and demonstrated localization of MSCs in spinal ligaments. Immunolocalization of MSCs was observed in the perivascular area and collagenous matrix in spinal ligaments. We also isolated and expanded MSCs from human skin tissue. We showed that the osteogenic differentiation potential was significantly higher in MSCs from OPLL patients than in those from non-OPLL patients in both spinal ligament and skin tissues. Osteogenic-related gene expressions were significantly higher in the OPLL group than in the non-OPLL group. These findings suggest an increase in the osteogenic differentiation potential of MSCs from OPLL patients and that this propensity toward the osteogenic lineage may be a causal factor in the ossification in these ligaments.
|
Research Products
(8 results)
-
-
[Journal Article] Genetic Study Group of Investigation Committee on Ossification of the Spinal Ligaments,genome-wide sib-pair linkage analysis of ossification of the posterior longitudinal ligament of the spine2013
Author(s)
Karasugi T, Nakajima M, Ikari K; , Tsuji T, Matsumoto M, Chiba K, Uchida K, Kawaguchi Y, Mizuta H, Ogata N, Iwasaki M, Maeda S, Numasawa T, Abumi K, Kato T, Ozawa H, Taguchi T, Kaito T, Neo M, Yamazaki M, Tadokoro N, Yoshida M, Nakahara S, Endo K, Imagama S, Demura S, Sato K, Seichi A, Ichimura S, Watanabe M, Watanabe K, Nakamura Y, Mori K, Baba H, Toyama Y, Ikegawa S
-
Journal Title
J Bone Miner Metab
Volume: 31(2)
Pages: 136-43
-
[Journal Article] Mesenchymal stem cell isolation and characterization from human spinal ligaments2012
Author(s)
Asari T, Furukawa K, Tanaka S, Kudo H, Mizukami H, Ono A, Numasawa T, Kumagai G, Motomura S, Yagihashi S, Toh S
-
Journal Title
Biochem Biophys Res Commun
Volume: 417(4)
Pages: 1193-9
-
[Journal Article] Simple foot tapping test as a quantitative objective assessment of cervical myelopathy2012
Author(s)
Numasawa T, Ono A, Wada K, Yamasaki Y, Yokoyama T, Aburakawa S, Takeuchi K, Kumagai G, Kudo H, Umeda T, Nakaji S, Toh S
-
Journal Title
Spine (Phila Pa 1976).
Volume: 37(2)
Pages: 108-13
-
[Journal Article] Genetic differences in the osteogenic differentiation potency according to the classification of ossification of the posterior longitudinal ligament of the cervical spine2011
Author(s)
Kudo H, Furukawa K, Yokoyama T, Ono A, Numasawa T, Wada K, Tanaka S, Asari T, Ueyama K, Motomura S, Toh S
-
Journal Title
Spine (Phila Pa 1976)
Volume: 36(12)
Pages: 951-7
-
-
-