2013 Fiscal Year Final Research Report
An EGFR inhibitor induces Schwann cell proliferation and promotes functional recovery with remyelination in injured peroneal nerve after end-to-side neurorrhaphy
| Project/Area Number |
23592160
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ENOMOTO Mitsuhiro 東京医科歯大学, 医学部附属病院, 講師 (90451971)
OKAWA Atsushi 東京医科歯大学, 医歯学総合研究科, 教授 (30251507)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 神経再生 / 上皮成長因子受容体 / 神経栄養因子 / 末梢神経損傷 |
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| Research Abstract |
In this study, we tested whether the inhibition of epidermal growth factor receptor (EGFR) regulates neurite extension in DRG neurons or Schwann cell (SC) proliferation in vitro and nerve regeneration in injured proneal nerves after end-to-side neurorrhaphy (ETS). The in vitro results showed that regeneration of neurites from explanted DRG neurons was not significantly accelerated by administration of EGFR inhibitor (EGFI). In contrast, the number of SCs was increased with EGFI treatment as shown by a viability assay. Rat peroneal nerve was cut and then ETS was performed 3 weeks later. EGFI was infused by osmotic pump at the injury site for 7 days. After the surgery, motor score was increased significantly at 2 weeks and the amount of myelin in the peroneal nerve was increased significantly at 4 weeks in the EGFI infusion group. These findings suggest that local administration of EGFI has the potential to increase functional recovery after peripheral nerve injury.
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