Elucidation of the pain mechanisms in the extra-foraminal and double crush lesions of the L5 spinal nerve

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592174
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Wakayama Medical University
• Principal Investigator: HASHIZUME Hiroshi 和歌山県立医科大学, 医学部, 講師 (10326382)
• Co-Investigator(Kenkyū-buntansha): NAKATSUKA Terumasa 関西医療大学, 保健医療学部, 客員教授 (30380752)
• Project Period (FY): 2011 – 2013
• Keywords: 神経障害性疼痛 / 動物モデル / 後根神経節 / 脊髄後角 / パッチクランプ法 / 腰部脊柱管狭窄症 / 椎間孔部障害

Research Abstract

The lumbar radiculopathy rat model was created by ligating the dorsal root ganglia (DRG) of the L5 spinal nerve, 2 mm proximal to the DRG or 2 mm distal to the DRG, and the two sites of them (i.e. double crush) with 6.0 silk. We examined the degree of the radiculopathy using mechanical sensitivity, immunohistochemistry and in vivo patch-clamp recordings, 7 days after surgery. The rats injured the DRG was most sensitive, and the rats injured distal to the DRG were more sensitive than those rats injured proximal to the DRG in the behavioral study. The number of activated microglia in laminas I-II of the L5 segmental level and the amplitudes and frequencies of EPSC in the rats increased in the same order with the behavioral study. There was no significant difference between the double crush models and the DRG injured model. Moreover, we found that lumbar sympathectomy decreased the mechanical sensitivity and the frequencies of EPSC in the DRG injured model.

Research Products

• [Journal Article] A new electrophysiological method for the diagnosis of extraforaminal stenosis at L5-s1 (2014)
  • Author(s): Iwasaki H, Yoshida M, Yamada H, Hashizume H, Minamide A, Nakagawa Y, Kawai M, Tsutsui S
  • Journal Title: Asian Spine J Volume: 8(2) Pages: 145-9
  • DOI: 10.4184/asj.2014.8.2.145
  • Peer Reviewed
• [Journal Article] Reactive oxygen species enhance excitatory synaptic transmission in rat spinal dorsal horn neurons by activating TRPA1 and TRPV1 channels (2013)
  • Author(s): Nishio N, Taniguchi W, Sugimura YK, Takiguchi N, Yamanaka M, Kiyoyuki Y, Yamada H, Miyazaki N, Yoshida M, Nakatsuka T
  • Journal Title: Neuroscience Volume: 5:247 Pages: 201-12
  • DOI: 10.1016/j.neuroscience.2013.05.023
  • Peer Reviewed
• [Journal Article] Efficacy of novel minimally invasive surgery using spinal microendoscope for treating extraforaminal stenosis at the lumbosacral junction (2012)
  • Author(s): Yamada H, Yoshida M, Hashizume H, Minamide A, Nakagawa Y, Kawai M, Iwasaki H, Tsutsui S
  • Journal Title: J Spinal Disord Tech Volume: 25 Pages: 268-76
  • DOI: 10.1097/BSD.0b013e3182206dd3
  • Peer Reviewed
• [Journal Article] Distinct degree of radiculopathy at different levels of peripheral nerve injury (2012)
  • Author(s): Takiguchi N, Yoshida M, Taniguchi W, Hashizume H, Yamada H, Miyazaki N, Nishio N, Nakatsuka T
  • Journal Title: Mol Pain Volume: 26 Pages: 8:31
  • DOI: 10.1186/1744-8069-8-31
  • Peer Reviewed
• [Presentation] バクロフェンの脊髄運動ニューロンに対する興奮抑制メカニズム (2014)
  • Author(s): 阿部唯一,谷口亘,峰巨,山中学,曽根勝真弓,筒井俊二,橋爪洋,山田宏,中塚映政,吉田宗人
  • Organizer: 第43回日本脊椎脊髄病学会
  • Place of Presentation: 京都
  • Year and Date: 20140400
• [Presentation] アストロサイト由来伝達物質D-セリンは脊髄後角における痛覚刺激を増強する (2014)
  • Author(s): 曽根勝真弓,谷口亘,筒井俊二,山中学,西尾尚子,阿部唯一,峰巨,橋爪洋,山田宏,中塚映政,吉田宗人
  • Organizer: 第43回日本脊椎脊髄病学会
  • Place of Presentation: 京都
  • Year and Date: 20140400
• [Presentation] 脊髄後角TRPA1/TRPM8の活性化による痛覚伝達の変調:in vivoパッチクランプ法による解析 (2014)
  • Author(s): 山中学,谷口亘,曽根勝真弓,阿部唯一,峰巨,筒井俊二,橋爪洋,山田宏,中塚映政,吉田宗人
  • Organizer: 第43回日本脊椎脊髄病学会
  • Place of Presentation: 京都
  • Year and Date: 20140400
• [Presentation] ニコチン性アセチルコリン受容体の脊髄運動前角細胞におけるシナプス制御機構の解析 (2014)
  • Author(s): 峰巨,谷口亘,阿部唯一,山中学,曽根勝真弓,筒井俊二,橋爪洋,山田宏,中塚映政,吉田宗人
  • Organizer: 第43回日本脊椎脊髄病学会
  • Place of Presentation: 京都
  • Year and Date: 20140400
• [Presentation] ドパミン視床下部脊髄路は第3の下向性疼痛抑制系の作用を形成する (2014)
  • Author(s): 谷口亘,山中学,西尾尚子,曽根勝真弓,阿部唯一,峰巨,筒井俊二,橋爪洋,山田宏,中塚映政,吉田宗人
  • Organizer: 第43回日本脊椎脊髄病学会
  • Place of Presentation: 京都
  • Year and Date: 20140400
• [Presentation] 情動中枢前帯状皮質の活性化は脊髄後角で直接痛みを増強する (2014)
  • Author(s): 谷口亘,山中学,西尾尚子,曽根勝真弓,阿部唯一,峰巨,筒井俊二,橋爪洋,山田宏,中塚映政,吉田宗人
  • Organizer: 第43回日本脊椎脊髄病学会
  • Place of Presentation: 京都
  • Year and Date: 20140400
• [Presentation] 活性酸素による脊髄後角TRPA1,TRPV1を介した興奮性シナプス伝達増強の作用機序 (2014)
  • Author(s): 西尾尚子,谷口亘,山中学,曽根勝真弓,阿部唯一,峰巨,筒井俊二,橋爪洋,山田宏,中塚映政,吉田宗人
  • Organizer: 第43回日本脊椎脊髄病学会
  • Place of Presentation: 京都
  • Year and Date: 20140400
• [Presentation] 腰神経傷害部位の違いによる根性疼痛発現強度の検討 (2012)
  • Author(s): 瀧口登,谷口亘,橋爪洋,峰巨,阿部唯一,宮崎展行,西尾尚子,山田宏,中塚映政,吉田宗人
  • Organizer: 第5回日本運動器疼痛学会
  • Place of Presentation: 東京
  • Year and Date: 20121100
• [Presentation] 腰神経の傷害部位の違いは神経障害性疼痛の発現にいかなる影響を与えるか (2012)
  • Author(s): 瀧口登,谷口亘,山田宏,橋爪洋,宮崎展行,峰巨,中塚映政,吉田宗人
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 20121000
• [Presentation] Distinct degree of radiculopathy at different levels of peripheral nerve injury (2012)
  • Author(s): Takiguchi N, Yoshida M, Taniguchi W, Hashizume H, Miyazaki N, Nishio N, Nakatsuka T
  • Organizer: 42th Annual Meeting of Society for Neuroscience
  • Place of Presentation: New Orleans
  • Year and Date: 20121000
• [Presentation] 末梢神経傷害部位の違いから生じる根性疼痛の発現について (2012)
  • Author(s): 瀧口登,吉田宗人,谷口亘,杉村弥恵,西尾尚子,中塚映政:,,, 2012, 07
  • Organizer: 第34回日本疼痛学会
  • Place of Presentation: 熊本
  • Year and Date: 20120700
• [Presentation] 腰神経根傷害部位で根性疼痛の発現強度に差は生じるのか―動物モデルを用いた行動学,組織学,電気生理学的解析― (2012)
  • Author(s): 瀧口登,谷口亘,山田宏,橋爪洋,宮崎展行,峰巨,中塚映政,吉田宗人
  • Organizer: 第41回日本脊椎脊髄病学会
  • Place of Presentation: 久留米
  • Year and Date: 20120400