2013 Fiscal Year Final Research Report
Role of GOLPH3 and GOLPH3L in the proliferation of human rhabdomyosarcoma
| Project/Area Number |
23592195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
KOMIYA Setsuro 鹿児島大学, 医歯(薬)学総合研究科, 教授 (30178371)
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| Co-Investigator(Kenkyū-buntansha) |
SETOGUCHI Takao 鹿児島大学, 大学院医歯学総合研究科, 特任 准教授 (40423727)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 横紋筋肉腫 / GOLPH3 / GOLPH3L / 外科系臨床医学・整形外科学 |
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| Research Abstract |
GOLPH3 was originally identified by proteomic analyses of Golgi proteins localized in the trans-Golgi network. Recently, it was reported that GOLPH3 is up-regulated in various types of malignancies. In order to explore the function of GOLPH3 and its isoform, GOLPH3L, in the pathogenesis of rhabdomyosarcoma, we investigated the expression and knockdown effects of GOLPH3/GOLPH3L in human rhabdomyosarcoma. Western blot analysis and real-time PCR revealed that human rhabdomyosarcoma cell lines and biopsy specimens exhibited an increased expression of GOLPH3/GOLPH3L. GOLPH3/GOLPH3L knockdown by siRNA prevented the proliferation of human rhabdomyosarcoma cell lines. In addition, double-knockdown of GOLPH3/GOLPH3L also prevented the proliferation of rhabdomyosarcoma cell lines. Our findings improve the understanding of rhabdomyosarcoma pathogenesis and suggest that the knockdown of GOLPH3/GOLPH3L may be an effective treatment for rhabdomyosarcoma.
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