Investigation of apoptosis mechanism in chondrocytes for prevention of osteoarthritis

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592213
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: NISHIYAMA Takayuki 神戸大学, 医学(系)研究科(研究院), 医学研究員 (10379373)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Shinya 神戸大学, 医学部附属病院, 特命助教 (20437487) ; FUJISHIRO Takaaki 神戸大学, 医学部附属病院, 特命助教 (50448172) ; KANZAKI Niroyuki 神戸大学, 医学部附属病院, 医員 (30514632) ; KURODA Ryosuke 神戸大学, 医学研究科, 准教授 (80379362) ; KUROSAKA Masahiro 神戸大学, 医学研究科, 教授 (70170115) ; NISHIDA Kotaro 神戸大学, 医学部附属病院, 講師 (00379372)
• Project Period (FY): 2011 – 2013
• Keywords: EPA / 軟骨組織 / アポトーシス / 変形性関節症

Research Abstract

Objective: Oxidative stress has been reported to induce apoptosis and degeneration of chondrocytes.But the mechanism of EPA in apoptosis and degeneration of chondrocytes has been unknouwn. We evaluated that the function of EPA on apoptosis and degeneration of chondrocytes.Methods: The expression of MMPs were detected by real-time PCR, and apoptosis related proteins were detected by western blotting and FACS. C57BL/6J mice were used for detecting MMPs expression by immunohistochemistry.Results: EPA inhibited SNP-induced apoptosis of chondrocytes. EPA inhibited SNP-induced expressions of MMP3 and MMP13. The progression of OA was prevented by articular injection of EPA in vivo. Immunohistochemistry demonstrated that MMP3 and MMP13 expression were increased in DMM model. However, intra-articular injection of EPA inhibited the expressions of MMP13. Conclusion: The treatment of EPA can control the oxidative stress-induced OA progression. EPA may be a new therapeutic approach in OA therapy.

Research Products

• [Journal Article] PTEN regulates matrix synthesis in adult human chondrocytes under oxidative stress (2014)
  • Author(s): Iwasa K, Hayashi S, Fujishiro T, Kanzaki N, Hashimoto S, Sakata S, Chinzei N, Nishiyama T, Kuroda R, Kurosaka M
  • Journal Title: J Orthop Res Volume: 32 Pages: 231-7
• [Journal Article] Akt phosphorylation in human chondrocytes is regulated by p53R2 in response to mechanical stress (2012)
  • Author(s): Kawakita K, Nishiyama T, Fujishiro T, Hayashi S, Kanzaki N, Hashimoto S, Takebe K, Iwasa K, Sakata S, Nishida K, Kuroda R, Kurosaka M
  • Journal Title: Osteoarthritis Cartilage Volume: 20 Pages: 1603-9
• [Presentation] エイコサペンタエン酸は酸化ストレス依存性の軟骨細胞の変性、アポトーシスを抑制する (2013)
  • Author(s): 坂田周平、林申也、藤代高明、神崎至幸、橋本慎吾、岩佐賢二郎、鎮西伸顕、木原伸介、黒田良祐、黒坂昌弘題目
  • Organizer: 日本整形外科基礎学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20130000
• [Presentation] エイコサペンタエン酸は酸化ストレス依存性の軟骨細胞の変性、アポトーシスを抑制する (2013)
  • Author(s): 坂田周平、、林申也、藤代高明、神崎至幸、橋本慎吾、岩佐賢二郎、鎮西伸顕、木原伸介、黒田良祐、黒坂昌弘題目
  • Organizer: 日本軟骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 20130000
• [Presentation] Oxidative stress-induced chondrocytes apoptosis and matrix loss were inhibited by eicosapentaenoic acid (2013)
  • Author(s): Shuhei Sakata, Shinya Hayashi, Takaaki Fujishiro, Kohei Kawakita, Noriyuki Kanzaki, Shingo Hashimoto, Kenjiro Iwasa, Nobuaki Chinzei, Shinsuke Kihara, Takayuki Nishiyama, Ryosuke Kuroda, Masahiro Kurosaka
  • Organizer: OARSI meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 20130000