2014 Fiscal Year Final Research Report
Functional analysis of novel DAP12-associated receptor in osteoclasts.
| Project/Area Number |
23592214
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Keywords | 骨・軟骨代謝学 / 破骨細胞 / 骨吸収 / NFATc1 / ITAM / レクチン |
|---|
| Outline of Final Research Achievements |
Siglec-15 is DAP12 associated receptor, which is essential for the formation of functional osteoclast. In this research, we identified a Siglec-15-binding protein that interacts with cytoplasmic region of Siglec-15. Cytoplasmic region of Siglec-15 that associates this protein was essential for the function of Siglec-15. Chimeric molecules consisting of the extracellular region, transmembrane region with a K272A mutation, and the functional region of cytoplasmic tail of Siglec-15 and the cytoplasmic region of DAP12 restore bone resorption in osteoclasts from Siglec-15 knockout mice that were established in this research. These results strongly suggested that Siglec-15 is a critical DAP12-associated receptor in osteoclasts.
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| Free Research Field |
骨代謝学
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