2013 Fiscal Year Final Research Report
Functional analysis of the novel target gene of bone volume-regulating transcription factor Schnurri-3.
| Project/Area Number |
23592222
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
ISHIDOU Yasuhiro 鹿児島大学, 医歯(薬)学総合研究科, 准教授 (10300740)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Shingo 鹿児島大学, 大学院医歯学総合研究科, 准教授 (60353463)
KOMIYA Setsuro 鹿児島大学, 大学院医歯学総合研究科, 教授 (30178371)
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| Project Period (FY) |
2011 – 2013
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| Keywords | Shn3 / Hivep3 / Alg2 / 骨芽細胞分化 / 軟骨細胞分化 / Runx2 |
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| Research Abstract |
Osteoporosis is a result of unbalanced bone formation and resorption in adult bone. Although Schnurri 3 (Shn3) negatively regulates bone volume in adult mice, the underlying molecular mechanism are not fully understood. We searched for genes which expression level were affected by knockdown of Shn3 in osteoblasts by microarray approach, and found that Alg2 was induced by Shn3. Alg2 physically interacted with the osteoblast differentiation master regulator Runx2 to interfere its nuclear localization, which resulted in its suppressed transcriptional activity. In chondrocytes, Alg2 induced Creb3l2, which is a crucial gene in the important physiological endoplasmic reticulum stress during chondrogenesis, to support chondrocyte maturation. Our findings revealed a novel possibility that mannosyltransferase genes play crucial roles in gene transcription and expression in cells of bone and cartilage.
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[Journal Article] Human Immunodeficiency Virus Type I Enhancer Binding Protein 3 is Essential for the Expression of Asparagine-linked Glycosylation 2 in the Regulation of Osteoblast and Chondrocyte Differentiation2014
Author(s)
Imamura K, Maeda S, Kawamura I, Matsuyama K, Shinohara N, Yahiro Y, Nagano S, Setoguchi T, Yokouchi M, Ishidou Y, Komiya S
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Journal Title
J Biol Chem
Volume: 289
Pages: 9865-9879
Peer Reviewed
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[Journal Article] BMP signaling upregulates neutral sphingomyelinase 2 to suppress chondrocyte maturation via the Akt signaling pathway as a negative feedback mechanism2014
Author(s)
Kakoi H, Maeda S, Shinohara N, Matsuyama K, Imamura K, Kawamura I, Nagano S, Setoguchi T, Yokouchi M, Ishidou Y, Komiya S
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Journal Title
J Biol Chem
Volume: 289
Pages: 8135-8150
Peer Reviewed
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[Presentation] Asparagine-linked glycosylation 2 homolog, identified as a downstream target of Schnurri-3, cell-autonomously regulates differentiation of osteoblasts and chondrocytes2013
Author(s)
Imamura, Katsuyuki ; Maeda, Shingo ; Kawamura, Ichiro ; Yokouchi, Masahiro ; Ishidou, Yasuhiro ; Komiya, Setsuro
Organizer
Orthopaedic Research Society 2013 Annual Meeting
Place of Presentation
San Antonio, TX, USA
Year and Date
20130126-29
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