2013 Fiscal Year Final Research Report
Molecular mechanism of responses to stress in blood coagulation and fibrinolysis system
| Project/Area Number |
23592249
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Gifu University |
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Principal Investigator |
AKAMATSU Shigeru 岐阜大学, 医学(系)研究科(研究院), 非常勤講師 (20167828)
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| Co-Investigator(Kenkyū-buntansha) |
KOZAWA Osamu 岐阜大学, 医学系研究科, 教授 (90225417)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 心血管 / 血栓 / 血小板 / 凝固 / 綿溶 |
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| Research Abstract |
We previously demonstrated that ristocetin, an activator of GPIb/IX/V, induces release of sCD40L via TXA2 production from human platelets. In the present study, we investigated the effect of antithrombin-III (AT-III), an anticoagulant, on the ristocetin-induced GPIb/IX/V activation in human platelets. AT-III inhibited ristocetin-stimulated platelet aggregation. The ristocetin-induced production of 11-dehydro-TXB2, a stable metabolite of TXA2, and the release of sCD40L were suppressed by AT-III. AT-III also reduced the ristocetin-stimulated secretion of PDGF-AB. AT-III failed to affect the U46619-, a TP receptor agonist, induced levels of p38 MAP kinase phosphorylation or sCD40L release. AT-III reduced the binding of SZ2, a monoclonal antibody to the sulfated sequence in the alpha-chain of GPIb, to the ristocetin-stimulated platelets. These results strongly suggest that AT-III reduce ristocetin-stimulated release of sCD40L due to inhibiting TXA2 production in human platelets.
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[Journal Article] Involvement of Rac in thromboxane A2-induced human platelet activation : regulation of sCD40 ligand release and PDGF-AB secretion2014
Author(s)
Kageyama Y, Doi T, Matsushima-Nishiwaki R, Iida Y, Akamatsu S, Kondo A, Kuroyanagi G, Yamamoto N, Mizutani J, Otsuka T, Tokuda H, Iida H, Kozawa O and Ogura S
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Journal Title
Mol. Med. Rep.
Volume: 10
Pages: 107-112
Peer Reviewed
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[Journal Article] Thrombopoietin amplifies ADP-induced HSP27 phosphorylation in human platelets : importance of its pre-treatment2013
Author(s)
Cuong TN, Doi T, Matsushima-Nishiwaki R, Akamatsu S, Kuroyanagi G, Kondo A, Mizutani J, Wada I, Otsuka T, Tokuda H, Kozawa O and Ogura S
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Journal Title
Int. J. Mol. Med.
Volume: 31
Pages: 1291-1297
Peer Reviewed
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[Journal Article] Effect of antithrombin III on glycoprotein Ib/IX/V activation in human platelets : suppression of thromboxane A2 generation2013
Author(s)
Doi T, Tokuda H, Matsushima-Nishiwaki R, Cuong NT, Kageyama Y, Iida Y, Kondo A, Akamatsu S, Otsuka T, Iida H, Kozawa O and Ogura S
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Journal Title
Prostaglandins Leukot. Essent. Fatty Acids
Volume: 87
Pages: 57-62
Peer Reviewed
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[Journal Article] Time to initiation of treatment with polymyxin B cartridge hemoperfusion in septic shock patients2012
Author(s)
Takeyama N, Noguchi H, Hirakawa A, Kano H, Morino K, Obata T, Sakamoto T, Tamai F, Ishikura H, Kase Y, Kobayashi M, Naka T, Takahashi Y, Japan Sepsis Study Group (Akamatsu S)
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Journal Title
Blood Purif
Volume: 33
Pages: 252-256
Peer Reviewed
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[Journal Article] cAMP regulates ADP-induced HSP27 phosphorylation in human platelets2011
Author(s)
Enomoto Y, Adachi S, Doi T, Natsume H, Kato K, Matsushima-Nishiwaki R, Akamatsu S, Tokuda H, Yoshimura S, Otsuka T, Ogura S, Kozawa O and Iwama T
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Journal Title
Int. J. Mol. Med.
Volume: 27
Pages: 695-700
Peer Reviewed
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