Analysis of the molecular mechanism of the interaction of heterodimerized m-opioid and CB1 receptors

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592299
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Nagasaki University
• Principal Investigator: HOJO Minoru 長崎大学, 医歯薬学総合研究科(医学系), 助教 (00380957)
• Co-Investigator(Kenkyū-buntansha): UEZONO Yasuhito 国立がん研究センター, 研究所, 分野長 (20213340)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: オピオイド / カナビノイド / 鎮痛 / 二量体

Outline of Final Research Achievements

The marijuana is known as a drug producing for a long time. Cannabinoid CB1 which is the chief ingredient of the marijuana is used in multiple sclerosis and AIDs, cancer patients even now. To visually and biochemically demonstrate the heterodimerization of mOR and CB1R, we carried out confocal microscopy and fluorescence resonance energy transfer (FRET), and immunoprecipitation
followed by Western blot analysis. We also examined signal transduction pathways activated by dimerized mOR/CB1R with the electrophysiological assay of G protein-activated inwardly rectifying K+ (GIRK) channels and the measurement of total and phosphorylated extracellular signal-regulated kinases (ERK).

Free Research Field

麻酔科学