New treatment strategy of bladder cancer indexed by centrosome amplification

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592333
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Yamaguchi University
• Principal Investigator: MATSUYAMA Hideyasu 山口大学, 医学(系)研究科(研究院), 教授 (70209667)
• Co-Investigator(Kenkyū-buntansha): SASAKI Kohsuke 山口大学, 大学院医学系研究科, 教授 (80116722) ; HARA Takahiko 山口大学, 医学部附属病院, 助教 (70511715)
• Project Period (FY): 2011 – 2013
• Keywords: 膀胱癌 / 中心体複製異常 / 薬剤感受性 / パクリタキセル

Research Abstract

The aims of this study were to identify the relevant gene of centrosome amplification (CA) and its regulating microRNA (miR) in bladder cancer, to establish the CA-positive from CA-negative (KK-47) bladder cancer cell line by the gene transfer technique, and to compare the invasive phenotype, or sensitivity of paclitaxel between parent and gene-transferred cell line. To pick-up the relevant genes to CA, array CGH technique was applied and referred the open miR database to find the relevant miR regulating such genes. As a result, hsa-miR-1225-5p and GBAS was selected as miR and relevant gene to control CA, respectively. The GBAS transferred KK-47 cell lined showed significantly higher cell population with CA than parent KK-47 cell lines. Comparison of invasive phenotype, sensitivity of paclitaxel between parent and gene-transferred cell line are under investigation.

Research Products

• [Journal Article] Copy number aberrations using multicolour fluorescence in situ hybridization (FISH) for prognostication in non-muscle-invasive bladder cancer (NIMBC) (2014)
  • Author(s): Matsuyama H, Ikemoto K, Eguchi S, Oga A, Kawauchi S, Yamamoto Y, Kawai Y, Matsumoto H, Hara T, Nagao K, Sakano S, Sasaki K
  • Journal Title: BJU Int Volume: 113 Pages: 7-662
  • Peer Reviewed
• [Journal Article] Centrosome amplification in bladder washing cytology specimens is a useful prognostic biomarker for non-muscle invasive bladder cancer (2013)
  • Author(s): Miyachika Y, Yamamoto Y, Matsumoto H, Nishijima J, Kawai Y, Nagao K, Hara T, Sakano S, Matsuyama H
  • Journal Title: Cancer Genet Volume: 206 Pages: 8-12
  • Peer Reviewed
• [Journal Article] DNA copy number aberrations associated with Lymphovascular invasion in upper urinary tract urothelial carcinoma (2012)
  • Author(s): Misumi, T, Yamamoto Y, Miyachika Y, Eguchi S, Chochi Y, Nakao M, Nagao K, Hara T, Sakano S, Furuya T, Oga A, Kawauchi S, Sasaki K, Matsuyama H
  • Journal Title: Cancer Genet Volume: 205 Pages: 8-313
  • Peer Reviewed
• [Journal Article] Centrosome amplification as a putative prognostic biomarker for the classification of urothelial carcinomas (2011)
  • Author(s): Yamamoto Y, Misumi T, Eguchi S, Chochi Y, Kitahara S, Nakao M, Nagao K, Hara T, Sakano S, Furuya T, Oga A, Kawauchi S, Sasaki K, Matsuyama H
  • Journal Title: Hum Pathol Volume: 42 Pages: 30-1923
  • Peer Reviewed
• [Journal Article] Multicolor-FISHを用いた尿路上皮癌の診断とgrade分類の基礎的検討 (2011)
  • Author(s): 池本健三.江口賢.古屋智子.小賀厚徳.河内茂人.松山豪泰.佐々木功典
  • Journal Title: Cytometry Res Volume: 21 Pages: 65-70
• [Journal Article] 筋層非浸潤性膀胱癌の予後(総説) (2011)
  • Author(s): 松山豪泰
  • Journal Title: 西日泌尿 Volume: 73 Pages: 538-547
• [Presentation] Centrosome amplification (CA) leads chromosomal instability (CIN) in non-muscle invasive bladder cancer (2013)
  • Author(s): Matsuyama, H
  • Organizer: Korean Urological Association annual meeting
  • Place of Presentation: Seoul,Korea
  • Year and Date: 2013-11-29
• [Presentation] Clinical significance and cytogenetic analysis of false positive mucosa of the photodynamic diagnosis (PDD)using 5-aminolevlinic acid (5-ALA) (2013)
  • Author(s): Matsuyama, H
  • Organizer: East Asian GU cancer meeting
  • Place of Presentation: Seoul,Korea
  • Year and Date: 2013-03-29
• [Presentation] 膀胱洗浄細胞診検体を用いたmulti-color FISHによる膀胱癌の診断と予後予測の試み (2012)
  • Author(s): 松山豪泰、池本健三、江口賢、佐々木功典
  • Organizer: 第53回日本臨床細胞学会総会
  • Place of Presentation: 幕張メッセ国際会議場,千葉
  • Year and Date: 2012-06-02
• [Presentation] Centrosome amplification in bladder washing cytology samples may become prognostic marker in non-muscle invasive bladder cancer (2012)
  • Author(s): Miyachika, Y., Yamamoto, Y., Matsumoto, H., Nishijima, J., Kawai, Y., Hara, T., Sakano, S., Matsuyama, H
  • Organizer: AUA annual meeting
  • Place of Presentation: Atlanta,USA
  • Year and Date: 2012-05-22
• [Remarks] 松山豪泰:膀胱癌.今日の臨床サポート.永井良三,福井次矢,木村健二郎,上村直実, 桑島巌,今井靖,嶋田元,編
  • URL: http://clinicalsup.jp/jpoc/