Comprehensive gene analysis of false positive urothelium in photodynamic diagnosis to explore the novel carcinogenesis-related gene

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592335
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Kochi University
• Principal Investigator: INOUE Keiji 高知大学, 教育研究部医療学系, 准教授 (00294827)
• Co-Investigator(Kenkyū-buntansha): TAMURA Kenji 高知大学, 教育研究部医療学系, 助教 (50464384) ; SHUIN Taro 高知大学, 教育研究部医療学系, 教授 (70128601) ; OGURA Shunichiro 東京工業大学, 生命理工学研究科, 准教授 (90343160)
• Project Period (FY): 2011 – 2013
• Keywords: 膀胱癌 / 光力学診断 / アミノレブリン酸 / 偽陽性病変 / 遺伝子解析

Research Abstract

Comprehensive gene analysis of false positives in aminolevulinic acid-induced photodynamic diagnosis were performed using biopsy specimen of urinary bladder cancer in this study. As a result of measuring the level of mRNA obtained from positive fluorescent cancerous lesion and negative fluorescent non-cancerous lesion, high expression of peptide transporter PEPT1 and low expression of human ATP-binding cassette trans-porter ABCG2 were demonstrated the responsible genes for cancer-specific overaccumulation. Moreover, as a result of mass analysis by high performance liquid chromatography, the precursors of PpIX, uroporphyrin and coproporphyrin, were overexcreted to urine by the cancer-specific abnormal activity of these PpIX-related transporters.

Research Products

• [Journal Article] Expression levels of PEPT1 and ABCG 2 play key roles in aminolevulinic acid (ALA)-induced tumor-specific p rotoporphyrin IX (PpIX) accumulatio n in bladder cancer (2013)
  • Author(s): Hagiya Y, Fukuhara H, Matsumoto K, Endo Y, Nakajima M, Tanaka T, Okura I, Kurabayashi A, Furihata M, Inou e K, Shuin T, Ogura S
  • Journal Title: Photodiagnosis and Photodynamic Therapy Volume: 10 (3) Pages: 95-288
  • DOI: 10.1016/j.pdpdt.2013.02.001
  • Peer Reviewed
• [Journal Article] Porphyrins as urinary biomarkers fo r bladder cancer after 5-aminolevul inic acid administration : The poten tial of photodynamic screening for tumors (2013)
  • Author(s): Inoue K, Ota U, Ishizuka M, Kawada C, Fukuhara H, Shuin T, Okura I, Ta naka T, Ogura S
  • Journal Title: Photodiagnosis and Photodynamic The rapy Volume: 10 (4) Pages: 484-489
  • DOI: 10.1016/j.pdpdt.2013.05.002
  • Peer Reviewed
• [Journal Article] 膀胱癌診療における光力学診断(PDD)およびスクリーニング(PDS)の有用性、シンポジウムIII「膀胱癌の再発予防について」 (2013)
  • Author(s): 井上啓史,福原秀雄,川田千明,執印太郎,倉林睦,降幡睦夫,小倉俊一郎,石塚昌宏,高橋究,太田麗,石井琢也,田中徹
  • Journal Title: 日本腎泌尿器疾患予防医学研究会雑誌 Volume: 21(1) Pages: 48-51
• [Presentation] 5-アミノレブリン酸(ALA)による光力学技術を用いた対癌新戦略 (2013)
  • Author(s): 井上啓史
  • Organizer: ランチョンセミナー.第22回日本がん転移学会学術集会
  • Place of Presentation: 長野県松本市ホテルブエナビスタ
  • Year and Date: 2013-07-12
• [Presentation] 5-アミノレブリン酸(ALA)による光力学技術を用いた対癌新戦略 (2013)
  • Author(s): 井上啓史、福原秀雄、執印太郎、倉林睦、降幡睦夫、渡邊裕修、谷村正信、小倉俊一郎、石塚昌宏、田中徹
  • Organizer: 日本農芸化学会2013年度大会シンポジウム4SY18-8生命の根源物質アミノレブリン酸が拓く、健康・農業・医療への多様な応用の最前線
  • Place of Presentation: 宮城県仙台市東北大学川内北キャンパス
  • Year and Date: 2013-03-27
• [Presentation] 膀胱癌診療における光力学診断(PDD)およびスクリーニング(PDS)の有用性 (2012)
  • Author(s): 井上啓史、福原秀雄、執印太郎、倉林睦、降幡睦夫、小倉俊一郎、石塚昌宏、田中徹
  • Organizer: 第21回日本腎泌尿器疾患予防医学研究会シンポジウムIII～膀胱癌の再発予防について～
  • Place of Presentation: 高知県高知市文化プラザかるぽーと
  • Year and Date: 2012-07-13
  • Invited