2013 Fiscal Year Final Research Report
Castration-resistant prostate cancer-specific protease: Function analysis and application search as a therapeutic target.
| Project/Area Number |
23592338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kumamoto University |
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Principal Investigator |
WADA Yoshihiro 熊本大学, 大学院生命科学研究部, 准教授 (20284755)
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| Co-Investigator(Kenkyū-buntansha) |
IMAMURA Takahisa 熊本大学, 大学院生命科学研究部, 准教授 (20176499)
KAWANO Yoshiaki 熊本大学, 大学院生命科学研究部, 助教 (30593793)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 前立腺癌 / 去勢不応答 / プロテアーゼ / 細胞膜 |
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| Research Abstract |
Protease activities in culture media of prostate cancer cells were measured using 95 dipeptidyl substrates and a hydrolytic activity for Ac-XZ-MCA was found in media of castration-resistant but not -sensitive type cells. This activity was completely inhibited by DFP but not by other inhibitors, indicating this protease is serine-type. This activity was much higher in the washed cell suspensions than the culture supernatants and was localized on the cancer cell membrane when stained using biotin-XZ-chloromethylketone, which revealed that this protease is not secreted to the media but bound to the cell membrane. This protease was separated from the cell membrane and subjected to proteomics analysis but has not been identified because of a small amount of the protease. This castration-resistant prostate cancer cell-specific protease is a promising therapeutic target, thus further purification procedures will be done for its identification.
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Nitta, H., Wada, Y., Kawano, Y., Murakami, Y., Irie, A., Taniguchi, K., Kikuchi, K., Yamada, G., Suzuki, K., Honda, J., Wilson-Morifuji, M., Araki, N., Eto, M., Baba, H. and Imamura, T.
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DOI
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FEBS Letters
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Author(s)
前田喜寛,和田孝浩,河野吉昭,菊池健,高橋渡,杉山豊,田上憲一郎,矢津田旬二,今村隆寿,江藤正俊
Organizer
第72回日本癌学会学術総会
Place of Presentation
横浜市,パシフィコ横浜
Year and Date
20131003-05
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[Presentation] 転移性腎細胞癌におけるC5a受容体の発現および生物学的意義について2013
Author(s)
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Organizer
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Place of Presentation
高知市
Year and Date
20130308-09
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[Presentation] Aberrant expression of C5aR in metastatic renal cell carcinoma2012
Author(s)
MAEDA, Y., Wada, Y., Kawano, Y., Kikuchi, K., Takahashi, W., Honda, J., Nakanishi, J., Imamura, T., Eto, M.
Organizer
AUA Annual Meeting 2012
Place of Presentation
横浜市,パシフィコ横浜
Year and Date
20120519-23
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