Analysis of gene regulation networks controlled by microRNA in castration resistant prostate cancer

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592351
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Teikyo University
• Principal Investigator: KOJIMA Satoko 帝京大学, 医学部, 准教授 (10345019)
• Co-Investigator(Kenkyū-buntansha): SEKI Naohiko 千葉大学, 大学院医学研究院, 准教授 (50345013)
• Project Period (FY): 2011 – 2013
• Keywords: 前立腺癌 / マイクロRNA / 標的遺伝子 / 発現プロファイル / ゲノム / 増殖 / 浸潤

Research Abstract

Prostate cancer progress to castration resistant prostate cancer (CRPC) leading to the cancer death. Most of the patients who died with CRPC was initially revealed advanced disease with bone metastasis. We had analyzed the expression profiles of micro-RNAs in advanced prostate cancer. From 2011 to 2012, we confirmed the expression profile of microRNA of advanced prostate cancer. We had further analyzed the functions of down-regulated microRNAs in prostate cancer.
a) miR-145 target FASN1 and controls proliferation, invasion and migration functions of prostate cancer. b) miR a and miR-133a target PNP and controls proliferation, invasion and migration functions of prostate cancer. c) miR-222 and miR-31 involved cancer pathway (by KEGG pathway). d) miR-143/145 forms cluster and target GOLM1 which affect proliferation, invasion and migration functions of prostate cancer.

Research Products

• [Journal Article] The tumor-suppressive microRNA-143/145 cluster inhibits cell migration and invasion by targeting GOLM1 in prostate cancer (2014)
  • Author(s): Satoko Kojima, Hideki Enokida, Hirofumi Yoshino, Toshihiko Itesako, Takeshi Chiyomaru, Takashi Kinoshita, Miki Fuse, Rika Nishikawa, Yusuke Goto, Yukio Naya, Masayuki Nakagawa and Naohiko Seki
  • Journal Title: J Hum Genet Volume: 59(2) Pages: 78-87
  • DOI: 10.1038/jhg
  • Peer Reviewed
• [Journal Article] Tumor-suppressive microRNA-143/145 cluster targets hexokinase-2 in renal cell carcinoma (2013)
  • Author(s): Hirofumi Yoshino, Hideki Enokida, Toshihiko Itesako, Satoko Kojima, Takashi Kinoshita, Shuichi Tatarano, Takeshi Chiyomaru, Masayuki Nakagawa and Naohiko Seki
  • Journal Title: Cancer Sci Volume: 104(12) Pages: 1567-74
  • DOI: 10.1111/cas.12280
  • Peer Reviewed
• [Journal Article] Epithelial-mesenchymal transition-related microRNA-200s regulate molecular targets and pathways in renal cell carcinoma (2013)
  • Author(s): Hirofumi Yoshino, Hideki Enokida, Toshihiko Itesako, Shuichi Tatarano, Takashi Kinoshita, Miki Fuse, Satoko Kojima, Masayuki Nakagawa and Naohiko Seki
  • Journal Title: J Hum Genet Volume: 58(8) Pages: 508-16
  • DOI: 10.1038/jhg.2013.31
  • Peer Reviewed
• [Journal Article] Tumor suppressive microRNAs (miR-222 and miR-31) regulate molecular pathways based on microRNA expression signature in prostate cancer (2012)
  • Author(s): Miki Fuse, Satoko Kojima, Hideaki Enokida, Takeshi Chiyomaru, Hiroshi Yoshino, Nijiro Nohata, Takashi Kinoshita, Shinich Sakamoto, Yukio Naya, Masayuki Nakagawa, Tomohiko Ichikawa, Naohiko Seki
  • Journal Title: J Hum Genet Volume: 57(11) Pages: 691-699
  • DOI: 10.1038/jhg.2012.95
  • Peer Reviewed
• [Journal Article] Tumor suppressors miR-1 and miR-133a target the oncogenic function of purine nucleoside phosphorylase (PNP) in prostate cancer (2012)
  • Author(s): Satoko Kojima, Takeshi Chiyomaru, Kazumori Kawakami, Hirofumi, Yoshino, Hideki Enokida, Nijiro Nohata, Miki Fuse, Tomohiko Ichikawa, Yukio Naya, Masayuki Nakagawa and Naohiko Seki
  • Journal Title: Brit. J. Can Volume: 106 Pages: 405-413
  • DOI: 10.1038/bjc.2011.462
  • Peer Reviewed
• [Journal Article] Mir-96 and miR-183 detection in urine serve as potential tumor markers of urothelial carcinoma : correlation with stage and grade, and comparison with urinary cytology (2011)
  • Author(s): Yasutoshi Yamada, Hideki Enokida, Satoko Kojima, Kazumori Kawakami, Takeshi Chiyomaru, Shuichi Tatarano, Hirofumi Yoshino, Kazuya Kawahara, Kenryu Nishiyama, Naohiko Seki and Masayuki Nakagawa
  • Journal Title: Cancer Sci Volume: 102(3) Pages: 522-529
  • DOI: 10.1111/j.1349-7006.2010.01816.x
  • Peer Reviewed
• [Journal Article] Restoration of miR-145 expression suppresses cell proliferation, migration and invasion in prostate cancer by targeting FSCN1 (2011)
  • Author(s): Miki Fuse, Nijiro Nohata, Satoko Kojima, Shinichi Sakamoto, Takeshi Chiyomaru, Kazumori Kawakami, Hideki Enokida, Masayuki Nakagawa , Yukio Naya, Tomohiko Ichikawa and Naohiko Seki
  • Journal Title: Int. J. Oncol Volume: 38 Pages: 1093-1101
  • DOI: 10.3892/ijo.2011.919
  • Peer Reviewed
• [Presentation] PSA/T ratio is a prognostic factor to predict PSA recurrence in prostate cancer treated with intermittent androgen suppression (2013)
  • Author(s): Satoko Kojima, Hiroshi, Masuda, Kazuhiro Araki, Masahiko Inahara, Masahiso Sugiura, Kyokusin Hou, Naohide Sato, Yukio Naya
  • Organizer: SIU (33^<rd> congress of the société internationalďurology) 2013
  • Place of Presentation: Vancouver(UP-254)
  • Year and Date: 20130908-12
• [Presentation] Expression signature of tumor-suppressive miRNA and control of cancer associated molecular pathways in prostate cancer (2013)
  • Author(s): Satoko Kojima, Hideki Enokida, Hirohumi Yoshino, Takeshi Chiyomaru, Takashi Kinoshita, Miki Fuse, Yukio Naya, Masayuki Nakagawa, Naohiko Seki
  • Organizer: 第104回AUA Annual Meeting 2013
  • Place of Presentation: San Diego
  • Year and Date: 20130504-08
• [Presentation] Identification of pureine nucleoside phosphorylase (PNP) as a novel target of tumor suppressors miR-1 and MiR-133a in prostate cancer (2012)
  • Author(s): Satoko Kojima, Takeshi Chiyomaru, Hirofumi Yoshino, Hideki Enokida, Nijiro Nohata, Miki Fuse, Tomohiko Ichikawa, Yukio Naya, Masayuki Nakagawa, and Naohiko Seki
  • Organizer: 第103回AUA annual meeting
  • Place of Presentation: Atlanta, GA USA(Abstract No 487)
  • Year and Date: 20120519-23
• [Presentation] Tumor suppressors miR-1 and miR-133a target the oncogenic function of purine nucleoside phosphorylase (PNP) in prostate cancer (2012)
  • Author(s): Satoko Kojima, Takeshi Chiyomaru, Hideki Enokida, Kazumori Kawakami, Hirofumi Yoshino, Nijiro Nohata, Miki Fuse, Tomohiko Ichikawa, Yukio Naya, Masayuki Nakagawa, and Naohiko Seki
  • Organizer: 27^<th> EAU
  • Place of Presentation: Paris(abstract No 842)
  • Year and Date: 20120224-08
• [Presentation] 前立腺癌におけるmicro-RNA発現プロファイルと遺伝子制御の探索 (2012)
  • Author(s): 小島聡子、布施美樹、千代丸剛、野畑二次郎、木下崇、吉野裕史、榎田英樹、中川昌之、市川智彦、納谷幸男、関直彦
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-21
• [Presentation] 前立腺癌におけるmiR-1およびmiR-133aの機能解析と標的遺伝子PNPの同定 (2011)
  • Author(s): 小島聡子、千代丸剛、川上一盛、榎田英樹、野畑二次郎、布施美樹、吉野裕史、稲原昌彦、中川昌之、納谷幸男、関直彦
  • Organizer: 第70回日本癌学会学術集会
  • Place of Presentation: 京都(P-2101)
  • Year and Date: 20111003-05