2013 Fiscal Year Final Research Report
Evaluation of bladder and sexual function in rat water avoidance stress model.
| Project/Area Number |
23592367
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Osaka University |
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Principal Investigator |
TAKAO Tetsuya 大阪大学, 医学(系)研究科(研究院), 講師 (30379177)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIMURA Akira 大阪大学, 大学院医学系研究科, 准教授 (40294053)
MIYAGAWA Yasushi 大阪大学, 大学院医学系研究科, 講師 (70362704)
NONOMURA Norio 大阪大学, 大学院医学系研究科, 教授 (30263263)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ストレス / 頻尿 |
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| Research Abstract |
Water avoidance stress (WAS) is a potent psychological stressor and it is associated with visceral hyperalgesia. We investigated the voiding pattern and cyclooxygenase-2 (Cox-2) expression in a rat bladder model of WAS. Metabolic cage analysis and cystometrography showed significantly shorter intervals and less volume of voiding in WAS rats. Significantly higher expression of Cox-2 mRNA was verified by reverse transcription PCR. Immunohistochemistry and western blotting showed significantly higher Cox-2 protein levels in WAS bladders. Furthermore, immunohistochemistry showed high Cox-2 expression exclusively in smooth muscle cells. All WAS-induced changes were reduced by Cox-2 inhibitor pretreatment. Chronic stress might cause frequency through Cox-2 gene upregulation in bladder smooth muscle cells. Further study of Cox-2 in the WAS bladder might provide novel therapeutic modalities for interstitial cystitis.
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[Journal Article] Water avoidance stress induces frequency through cyclooxygenase-2 expression : a bladder rat model2012
Author(s)
Yamamoto K, Takao T, Nakayama J, Kiuchi H, Okuda H, Fukuhara S, Yoshioka I, Matsuoka Y, Miyagawa Y, Tsujimura A, Nonomura N
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Journal Title
Int J Urol
Volume: 19(2)
Pages: 155-62
DOI
Peer Reviewed
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