Identification and functional analysis of cytokine signal transduction-related factors in human urethral rhabdosphincter

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592372
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Oita University
• Principal Investigator: MIMATA hiromitsu 大分大学, 医学部, 教授 (60219714)
• Co-Investigator(Kenkyū-buntansha): SATO Fuminori 大分大学, 医学部, 准教授 (30305049) ; SUMINO Yasuhiro 大分大学, 医学部, 講師 (30325716) ; MORI Kenichi 大分大学, 医学部, 助教 (00579013)
• Project Period (FY): 2011 – 2013
• Keywords: 外尿道括約筋 / 尿失禁 / サイトカイン

Research Abstract

We explored the identification of specifically expressed factors in human RS and examined the effect on RS satellite cell growth, differentiation and the mechanisms of intracellular signal transduction by those factors. TGF-beta highly increased in ECM of RS by immunohistochemistry. Moreover, we identified that TGF-beta could inhibit cell proliferation and differentiation of human RS satellite cells through Smad2/3 phosphorylation.In the experiments using rat, urethral fnctions significantly decreased with aging after child-birth, and it has been considered that aging after child-birth attenuated urethral support. On the other hand, transcripts of ECM in urethra were significantly decreased with aging, although there were no significant changes in those of inflammatory cytokines between young and aged groups. Reduction of ECM or chronic inflammatory state in urethra including RS with aging might attenuate urethral support system,and be a part of SUI in elderly people.

Research Products

• [Journal Article] Therapeutic effects of IGF-1 on stress urinary incontinence in rats with simulated childbirth trauma (2014)
  • Author(s): Sumino Y, Yoshikawa S, Mimata H, Yoshimura N
  • Journal Title: J Urol Volume: 191 Pages: 529-38
  • Peer Reviewed
• [Journal Article] The Vav3 oncogene enhances the malignant potential of prostate cancer cells under chronic hypoxia (2014)
  • Author(s): Hirai K, Nomura T, Yamasaki M, Inoue T, Narimatsu T, Chisato Nakada PD, Yoshiyuki Tsukamoto PD, Matsuura K, Sato F, Moriyama M, Mimata H
  • Journal Title: Urol Oncol Volume: 32 Pages: 101-9
  • Peer Reviewed
• [Journal Article] CLP1 links tRNA metabolism to progressive motor-neuron loss (2013)
  • Author(s): Hanada T, Weitzer S, Mair B, Bernreuther C, Wainger BJ, Ichida J, Hanada R, Orthofer M, Cronin SJ,Komnenovic V, Minis A, Sato F, Mimata H, Yoshimura A, Tamir I, Rainer J, Kofler R, Yaron A, Eggan KC, Woolf CJ, Glatzel M, Herbst R, Martinez J, Penninger JM
  • Journal Title: Nature Volume: 495 Pages: 474-480
  • Peer Reviewed
• [Journal Article] Regenerative medicine as a new therapeutic strategy for lower urinary tract dysfunction (2013)
  • Author(s): Sumino Y, Mimata H
  • Journal Title: Int J Urol Volume: 20 Pages: 670-5
  • Peer Reviewed
• [Journal Article] Targeting the Vav3 oncogene enhances docetaxel-induced apoptosis through the inhibition of androgen receptor phosphorylation in LNCaP prostate cancer cells under chronic hypoxia (2013)
  • Author(s): Nomura T, Yamasaki M, Hirai K, Inoue T, Sato R, Matsuura K, Moriyama M, Sato F, Mimata H
  • Journal Title: Mol Cancer Volume: 8(12) Pages: 27
  • Peer Reviewed
• [Journal Article] Myostatin inhibits proliferation of human urethral rhabdosphincter satellite cells (2012)
  • Author(s): Akita Y, Sumino Y, Mori K, Nomura T, Sato F, Mimata H
  • Journal Title: Int J Urol Pages: 522-9
  • Peer Reviewed
• [Journal Article] Use of procalcitonin in patients on chronic hemodialysis : procalcitonin is not related with increased serum calcitonin (2012)
  • Author(s): Mori K, Noguchi M, Sumino Y, Sato F, Mimata H
  • Journal Title: ISRN Urol Pages: 431859
  • Peer Reviewed
• [Journal Article] Left Transperitoneal Adrenalectomywith a Laparoendoscopic Single-Site SurgeryCombined Technique : InitialCaseReports (2011)
  • Author(s): Sumino Y, Nakano D, Mori K, Nomura T, Sato F and Mimata H
  • Journal Title: Case report in Med Pages: 651380
  • Peer Reviewed
• [Journal Article] Long-TermCryopreservationof Pyramidalis Muscle Specimens as a Source of Striated Muscle Stem Cells for Treatment of Post-Prostatectomy Stress Urinary Incontinence (2011)
  • Author(s): Sumino Y, Hirata Y, Hanada M, Akita Y, Sato F and Mimata H
  • Journal Title: Prostate Pages: 1225-30
  • Peer Reviewed
• [Presentation] Association of remodelling of extracellular matrix and urethral dysfunction inducing stress urinary incontinence in aged, multiparous rats (2013)
  • Author(s): Sumino Y, Yoshikawa S, Mimata H, Yoshimura N
  • Organizer: ICS 2013 (Annual Meeting of the International Continence Society)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20130825-30
• [Presentation] Insulin-like growth factor-1 as an important endogenous growth factor for the recovery from stress urinary incontinence in rats with simulated childbirth injury (2013)
  • Author(s): Sumino Y, Yoshikawa S, Mimata H, Yoshimura N
  • Organizer: AUA2013
  • Place of Presentation: San Diego CA, USA
  • Year and Date: 20130504-08
• [Presentation] Therapeutic effects of insulin-like growth factor-1 on stress urinary incontinence in rats with simulated child birth trauma injury (2012)
  • Author(s): Sumino Y, Yoshikawa S, Mimata H, Yoshimura N
  • Organizer: AUA
  • Place of Presentation: Atlanta GA, USA
  • Year and Date: 20120519-24
• [Presentation] 膣拡張による腹圧性尿失禁モデルラットにおけるInsulin-like growth actor-1(IGF-1)の治療効果 (2012)
  • Author(s): 住野泰弘、吉川悟、三股浩光,吉村直樹
  • Organizer: 第9回泌尿器科再建再生研究会
  • Place of Presentation: 湯布院
  • Year and Date: 2012-06-16
• [Presentation] 腹圧性尿失禁に対する外尿道括約筋自己再生を目指して (2011)
  • Author(s): 住野泰弘、三股浩光
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋(シンポジウム)
  • Year and Date: 20110421-24
• [Presentation] Insulin-like growth factor-1 (IGF-1) accelerates recovery from stress urinary incontinence in rats with simulated child birth trauma injury through Akt signal transduction pathway (2010)
  • Author(s): Sumino Y, Yoshikawa S, Mimata H, Yoshimura N
  • Organizer: ICS 2012 (Annual Meeting of the International Continence Society)
  • Place of Presentation: Peking, China
  • Year and Date: 20101017-20
• [Book] 泌尿器科基礎手術学泌尿器科手術の術前・術後管理.ベッドサイド泌尿器科学(改訂第4版) (2013)
  • Author(s): 三股浩光,佐藤文憲
  • Total Pages: 168-174
• [Book] 泌尿器科疾患:後腹膜腫瘍,後腹膜線維症.今日の治療指針.2012年版 (2012)
  • Author(s): 三股浩光(山口徹、北原光夫、福井次矢)
  • Total Pages: 944-945
  • Publisher: 医学書院.東京
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/hinyoki/