The systemic and basic study on the clinical application of the nuclear transfer method.

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592398
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: University of Yamanashi
• Principal Investigator: HIRATA Shuji 山梨大学, 総合研究部, 教授 (00228785)
• Co-Investigator(Kenkyū-buntansha): FUKASAWA Hiroko 山梨大学, 総合研究部, 助教 (60362068) ; SHODA Tomoko 山梨大学, 総合研究部, 助教 (50345716)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 体細胞核移植 / ドナー体細胞 / 卵細胞質 / ミトコンドリア / 初期発生 / IVM / 凍結保存

Outline of Final Research Achievements

The final destination of the present study is clinical application of the nuclear transferred embryonal stem (ntES) cells. The systemic and basic study on the "nt" was carried out to establish the methodology; "1) in vitro maturation (IVM) of the immature oocyte, 2) cryopreservation of the enucleated IVM oocyte, and 3) reconstruction of the cloned embryo using the enucleated IVM oocyte and donor nucleus. However, it was indicated that the early development rate of the cloned embryo was very low, and the rate should be improved for further investigation. Therefore, we study the factors related to the development thoroughly. As a result, the dynamics of the cytoplasm of the cloned embryo was important for the development. Further study revealed that the changes in the distribution of the mitochondria were a key factor.
From the present study, the strategy that improve the early development of the cloned embryo was established.

Free Research Field

生殖生物学