2013 Fiscal Year Final Research Report
Relaxin and immune abnormality in human reproductive failure
| Project/Area Number |
23592403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kobe University |
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Principal Investigator |
YAMADA Hideto 神戸大学, 医学(系)研究科(研究院), 教授 (40220397)
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| Co-Investigator(Kenkyū-buntansha) |
NAKABAYASHI Koji 神戸大学, 医学部附属病院, 講師 (80362789)
MAKIHARA Natsuko 神戸大学, 医学部附属病院, 助教 (30593991)
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| Project Period (FY) |
2011 – 2013
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| Keywords | リラキシン |
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| Research Abstract |
The study was conducted to evaluate the effects of human relaxin on apoptosis in the human trophoblast derived HTR-8/SV neo cell line, which is a possible model of human extravillous trophoblasts (EVTs). HTR-8/SV neo cells were treated with different doses of human recombinant (rH2) relaxin. RXFP1 and RXFP2 mRNA expression was observed in HTR-8/SV neo cells. Compared with untreated control cultures, treatment with rH2 relaxin, decreased TUNEL-positive rate in HTR-8/SV neo cells was observed. Western blot analysis revealed that treatment with rH2 relaxin decreased the expression of caspase-3 and cleaved PARP, but in contrast increased Bcl-2 expression in those cells. These results suggest that rH2 relaxin has anti-apoptotic effects on HTR8/SV neo cells by decreasing pro-apoptotic caspase-3 and cleaved PARP expression and up-regulating anti-apoptotic Bcl-2 expression.
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