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2013 Fiscal Year Final Research Report

Mechanisms of specific regulation of gonadotropn subunits expression by pulsatile GnRH stimulation

Research Project

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Project/Area Number 23592404
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionShimane University

Principal Investigator

KANASAKI Haruhiko  島根大学, 医学部, 講師 (10325053)

Co-Investigator(Kenkyū-buntansha) ORIDE Aki  島根大学, 医学部, 助教 (00423278)
Project Period (FY) 2011 – 2013
KeywordsGnRH / ゴナドトロピン
Research Abstract

PACAP and its receptor are expressed not only in the central nervous system, but also in peripheral organs and function to stimulate pituitary hormone synthesis and release as both hypothalamic-pituitary releasing factor as well as an autocrine-paracrine factor within the pituitary. PACAP stimulates the expression of the gonadotropin alpha, LH betand FSH beta subunits as well as the GnRH receptor and its own PACAP type I receptor (PAC1R) in gonadotropin-secreting pituitary cells. In turn, GnRH, which has been known as a crucial component of gonadotropin secretion, stimulates the expression of PACAP and PAC1R in gonadotrophs. In addition, PAC1R presents in GnRH-producing neuronal cells and PACAP modulates the functions of GnRH neurons. It is evident that PACAP plays a role in reproductive function through regulating gonadotropins directly or indirectly by modulating GnRH release.

  • Research Products

    (17 results)

All 2013 2012 2011 2010

All Journal Article (10 results) Presentation (6 results) Book (1 results)

  • [Journal Article] Expression of gonadotropin-inhibitory hormone receptors in mouse pituitary gonadotroph LβT2 cells and hypothalamic gonadotropin-releasing hormone-producing GT1-7 cells2013

    • Author(s)
      Sukhbaatar U, Kanasaki H, Mijiddorj T, Oride A, Miyazaki K
    • Journal Title

      Endocr J

      Volume: 61 Pages: 253-34

  • [Journal Article] Kisspeptin induces expression of gonadotropin-releasing hormone receptor in GnRH-producing GT1-7 cells overexpressing G protein-coupled receptor 542013

    • Author(s)
      Sukhbaatar U, Kanasaki H, Mijiddorj T, Oride A, Miyazaki K
    • Journal Title

      Gen Comp Endocrinol

      Volume: 194 Pages: 94-101

  • [Journal Article] Pituitary adenylate cyclase-activating polypeptide (PACAP) increases expression of the gonadotropin-releasing hormone (GnRH) receptor in GnRH-producing GT1-7 cells overexpressiong PACAP type I receptor2013

    • Author(s)
      Kanasaki H, Mijiddorj T, Sukhbaatar U, Oride A, Miyazaki K
    • Journal Title

      Gen Comp Endocrinol

      Volume: 193 Pages: 95-102

  • [Journal Article] Circulating kisspeptin and pituitary adenylate cyclase-activating polypeptide (PACAP) do not correlate with gonadotropin serum levels2013

    • Author(s)
      Kanasaki H, Purwana IN, Oride A, Mijiddorj T, Sukhbaatar U, Miyazaki K
    • Journal Title

      Gynecol Endocrinol

      Volume: 29 Pages: 583-587

  • [Journal Article] Possible role of PACAP and its PAC1 receptor in the differential regulation of pituitary LHβ- and FSHβ- subunit gene expression by pulsatile GnRH stimulation2013

    • Author(s)
      Kanasaki H, Purwana IN, Miyazaki K
    • Journal Title

      Biol Reprod

      Volume: 88 Pages: 1-5

  • [Journal Article] Prolonged stimulation with thyrotropin-releasing hormone and pituitary adenylate cyclase-activating polypeptide desensitize their receptor functions in prolactin-producing GH3 cells2013

    • Author(s)
      Mijiddorj T, Kanasaki H, Sukhbaatar U, Oride A, Purwana IN, Miyazaki K
    • Journal Title

      Mol Cell Endocrinol

      Volume: 365 Pages: 139-145

  • [Journal Article] Gonadotropin-inhibitory hormone inhibits GnRH-induced gonadotropin subunit gene transcriptions by inhibiting AC/cAMP/PKA-dependent ERK pathway in LβT2 cells2012

    • Author(s)
      Son YL, Ubuka T, Millar RP, Kanasaki H, Tsutsui K
    • Journal Title

      Endocrinology

      Volume: 153 Pages: 2332-2343

  • [Journal Article] Role of Neurokinin B and Dynorphin A in pituitary gonadotroph and somatolactotroph cell lines2012

    • Author(s)
      Mijiddorj T, Kanasaki H, Purwana IN, Oride A, Sukhbaatar U and Miyazaki K
    • Journal Title

      Endocr J

      Volume: 59 Pages: 631-640

  • [Journal Article] Effects of estradiol and progesterone on gonadotropin LHβ- and FSHβ-subunit promoter activities in gonadotroph LβT2 cells2012

    • Author(s)
      Kanasaki H, Purwana IN, Mijiddorj T, Sukhbaatar U, Oride A, Miyazaki K
    • Journal Title

      Neuro Endocrinol Lett

      Volume: 33 Pages: 608-613

  • [Journal Article] Effects of estradiol and progesterone on prolactin transcriptional activity in somatolactotrophic cells2012

    • Author(s)
      0Mijiddorj T, Kanasaki H, Purwana IN, Oride A, Sukhbaatar U and Miyazaki K
    • Journal Title

      Endocr J

      Volume: 59 Pages: 867-879

  • [Presentation] Prolonger stimulation with thyrotropin-releasing hormone and pituitary adenylate cyclase-activating polypeptide desensitize their receptor functions in prolactin-producing GH3 cells2013

    • Author(s)
      Haruhiko Kanasaki, Tselmeg Mijiddorj, Unurjargar Sukhbaatar, Aki Oride, Kohji Miyazaki
    • Organizer
      The Endocrine Society's 95^<th> annual meeting
    • Place of Presentation
      San Francisco, CA(USA)
    • Year and Date
      2013-06-17
  • [Presentation] Stimulatory effect of pituitary adenylate cyclase-activating polypeptide (PACAP) and its PACAP type I receptor (PAC1R) on prolactin synthesis in rat pituitary somatolactotroph GH3 cells2013

    • Author(s)
      Tselmeg Mijiddorj, Haruhiko Kanasaki, Unurjargar Sukhbaatar, Aki Oride, Kohji Miyazaki
    • Organizer
      The Endocrine Society's 95^<th> annual meeting
    • Place of Presentation
      San Francisco, CA(USA)
    • Year and Date
      2013-06-17
  • [Presentation] Circulating kisspeptin and pituitary adenylate cyclase-activating polypeptide do not correlate with gonadotropin serum levels2012

    • Author(s)
      Haruhiko Kanasaki, Aki Oride, Tselmeg Mijiddorj, Unurjargar Sukhbaatar, Kohji Miyazaki
    • Organizer
      2nd World Conferance Kisspeptin
    • Place of Presentation
      Tokyo
    • Year and Date
      20121106-09
  • [Presentation] Expression of the pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1R) potentiates the effects of GnRH on gonadotropin subunit gene expression2012

    • Author(s)
      Haruhiko Kanasaki, Aki Oride, Indri Purwana, Tselmeg Mijiddorj, Unurjargal Sukhbaatar, Kohji Miyazaki
    • Organizer
      The Endocrine Society's 94^<th> annual meeting
    • Place of Presentation
      Houston, TX (USA)
    • Year and Date
      2012-06-26
  • [Presentation] GnRH-induced PACAP and PAC1 Receptor Expression in Pituitary Gonadotrophs : A Possible Role in the Regulation of Gonadotropin Subunit Gene Expression2011

    • Author(s)
      Kanasaki H, Oride A, Purwana IN Mijiddorj T and Miyazaki K
    • Organizer
      The Endocrine Society's 93^<rd> annual meeting
    • Place of Presentation
      Boston, MA (USA)
    • Year and Date
      2011-06-24
  • [Presentation] Follistatin, induced by thyrotropin-releasing hormone (TRH), plays no role in prolactin expression but affects gonadotropin FSHb expression as a paracrine factor in pituitary somatolactotroph GH3 cells2010

    • Author(s)
      Kanasaki H, Oride A, Purwana IN, Miyazaki K
    • Organizer
      14^<th> International Congress of Endocrinology (ICE2010)
    • Place of Presentation
      Kyoto(Japan)
    • Year and Date
      20100326-30
  • [Book] Specific regulation of gonadotropin LHβ- and FSHβ-subunit gene expression by pulsatile GnRH stimulation. Gonadotropin-releasing hormone (GnRH): Production, Structure & Function2013

    • Author(s)
      Haruhiko Kanasaki, Aki Oride Kohji Miyazaki
    • Total Pages
      123-139
    • Publisher
      Nova Scientific Press, Inc (Ney York)

URL: 

Published: 2015-07-16  

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