2013 Fiscal Year Final Research Report
A new approach for pathogenesis and treatment of endometrosis using microarray analysis.
| Project/Area Number |
23592407
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NASU Kaei 大分大学, 医学部, 准教授 (30274757)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 子宮内膜症 / マイクロアレイ / エピジェネティクス / 増殖 / アポトーシス / 病態 |
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| Research Abstract |
We identified histone deacetylase 1-target mRNAs that were up-regulated by valpronic acid (VPA) treatment in endometriotic cyst stromal cells (ECSCs) by an mRNA microarray technique. We chose CCAAT/enhancer-binding protein alfa (C/EBP alfa) for further functional experiments. C/EBP alfa expression was attenuated in ECSCs and in endometriotic tissues. The compulsory expression of C/EBA alfa directed the inhibition of cell proliferation and the induction of apoptosis in ECSCs. C/EBP alfa knockdown directed the stimulation of cell proliferation and the resistance to apoptosis in normal endometrial cells. The expressions of various growth- and apoptosis-related molecules were down-regulated by C/EBP alfa knockdown. Our findings suggest that an epigenetically suppressed tumor suppressor gene is involved in the pathogenesis of endometriosis by creating the proliferative, anti-apoptotic, and other disease-specific characteristics of endometriosis.
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[Journal Article] Aberrant DNA methylation status of endometriosis: Epigenetics as the pathogenesis, biomarker, and therapeutic target2011
Author(s)
Nasu K, Kawano Y, Tsukamoto Y, Takano M, Takai N, Li H, Furukawa Y, Abe W, Moriyama M, Narahara H
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Journal Title
J Obstet Gynaecol Res
Volume: 37 (7)
Pages: 683-695
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[Presentation] Role of Mevalonate-Ras Homology (Rho)/Rho-associated Coiled-coil-forming Protein Kinase-mediated Signaling Pathway in the Pathogenesis of Endometriosis-associated Fibrosis2012
Author(s)
Nasu K, Tsuno A, Yuge A, Abe W, Kawano Y, Nishida M, Kai K, Matsumoto H, Kawano Y, Narahara H
Organizer
13th Royan International Congress on Reproductive Biomedicine
Place of Presentation
Tehran, Iran
Year and Date
20120905-07
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