Establishment and application of cancer-tissue originated spheroids(CTOSs) of gynecologic cancer

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592448
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: YOSHINO Kiyoshi 大阪大学, 医学(系)研究科(研究院), 講師 (90362730)
• Co-Investigator(Kenkyū-buntansha): ENOMOTO Takayuki (90283754) ; FUJITA Masami (60303963) ; UEDA Yutaka (10346215) ; KIMURA Toshihiro (90584524)
• Co-Investigator(Renkei-kenkyūsha): INOUE Masahiro (10342990)
• Project Period (FY): 2011 – 2013
• Keywords: 婦人科腫瘍学 / 初代培養 / 子宮体癌 / 漿液性腺癌 / 薬剤感受性 / ３次元培養 / CTOS / spheroid

Research Abstract

Drug sensitivity test for the patient of gynecology cancer by checking beforehand may give a merit. The aim of this study is establishment of a drug sensitivity examination using CTOS (cancer tissue-originated spheroid) which cultured the cancer cell extracted by an operation. As a result, it was revealed that among endometrial cancer, CTOS derived from the serous adenocarcinoma was prepared and maintained with high success rate. In addition, among the drugs which inhibit a signal related cancer growth, mTOR inhibitor, NF-kB inhibitor showed moderate activity.

Research Products

• [Journal Article] Second-line chemotherapy for advanced or recurrent endometrial carcinoma previously treated with paclitaxel and carboplatin, with or without epirubicin (2011)
  • Author(s): Ueda, Y. Miyake, T. Egawa-Takata, T. Miyatake, T. Matsuzaki, S. Yokoyama, T. Yoshino, K. Fujita, M. Enomoto, T. Kimura, T
  • Journal Title: Canc Chemot Volume: 67(4) Pages: 829-35
  • DOI: 10.1007/s00280-010-1384-z
  • Peer Reviewed
• [Journal Article] Recurrent endometrial carcinoma : prognosis for patients with recurrence within six to twelve months is worse relative at 12 months or later (2011)
  • Author(s): Miyake, T. Ueda, Y. Egawa-Takata, T. Matsuzaki, S. Yokoyama, T. Miyoshi, Y. Kimura, T. Yoshino, K. Fujita, M. Yamasaki, M. Enomoto, T. Kimura, T
  • Journal Title: Am J Obstet Gynecol Volume: 204(6) Pages: 535e-1-5
  • Peer Reviewed
• [Journal Article] Usefulness of computed tomography in predicting cytoreductive surgical outcomes for ovarian cancer (2011)
  • Author(s): Fujiwara, K. Yoshino, K. Enomoto, T. Fujita, M. Ueda, Y. Miyatake, T. Kimura, T. Muraji, M. Fujita, H. Kimura, T. Hori, M
  • Journal Title: Arch Gynecol Obstet Volume: 284(6) Pages: 1501-7
  • DOI: 10.1007/s00404-011-1864-3
  • Peer Reviewed
• [Journal Article] Chemotherapy for endometrial carcinoma(GOGO-EM1 study): TEC (paclitaxel, epirubicin and carboplatin) is an effective remission -induction and adjuvant therapy (2011)
  • Author(s): Egawa-Takata, T. Ueda, Y. Kuragaki, C. Miyake, T. Miyatake, T Fujita, M. Yoshino, K. Nakashima, R. Okazawa, M. Tsutsui, T. Morishige, K. Kimura, T. Yamasaki, M. Nshizawa, T. Nagamatasu, M. Ito, K. Asada, M. Ogita, K. Wakimoto, A. Yamamoto, T. Nishio, Y. Enomoto, T
  • Journal Title: Canc Chemot Volume: 68(6) Pages: 1603-10
  • DOI: 10.1007/s00280-011-1638-4
  • Peer Reviewed
• [Presentation] 子宮体癌に対する治療戦略;基礎と臨床 (2013)
  • Author(s): 吉野潔,難治性
  • Organizer: 第52回日本臨床細胞学会秋期大会
  • Place of Presentation: 大阪
  • Year and Date: 20131102-04
• [Presentation] CTOS培養法を用いた子宮体癌に対する抗癌剤感受性試験 (2013)
  • Author(s): 清原裕美子木村敏啓吉野潔平松宏祐松崎慎哉小林栄仁上田豊藤田征巳井上正宏榎本隆之木村正
  • Organizer: 第65回日本産科婦人科学会学術講演会
  • Place of Presentation: 札幌
  • Year and Date: 20130510-13
• [Presentation] Chemo-sensitivity test using cancer-tissue originated spheroids(CTOSs) of endometrial cancer (2012)
  • Author(s): Kiyohara, Y. Kimura, T. Yoshino, K. Morimoto, A. Ueda, Y. Fujita, M. Inoue, M. Enomoto, T. Kimura, T
  • Organizer: The 14th Biennial Meeting of the International Gynecologic Cancer Society
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 20121013-16
• [Presentation] Chemo-sensitivity test using cancer-tissue originated spheroids(CTOSs) of endometrial cancer (2012)
  • Author(s): Kiyohara, Y. Kimura, T. Yoshino, K. Morimoto, A. Ueda, Y. Fujita, M. Inoue, M. Enomoto, T. Kimura, T
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120919-21
• [Presentation] Establishment of cancer-tissue originated spheroids (CTOSs) and chemo-sensitivity test for endometrial cancer (2012)
  • Author(s): Kimura, T. Yoshino, K. Hiramatsu, K. Kiyohara, Y. Morimoto, A. Yokoyama, T. Kobayashi, E. Ueda, Y. Fujita, M. Inoue, M. Enomoto, T. Kimura, T
  • Organizer: The 103rd Annual Meeting American Association for Cancer Research
  • Place of Presentation: Chicago, U.S.A
  • Year and Date: 20120331-0404