new gene transduction to inner ear with confocal-micro endoscope

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592480
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kyoto University
• Principal Investigator: TAURA Akiko 京都大学, 医学(系)研究科(研究院), 助教 (70515345)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA Takayuki 京都大学, 大学院医学研究科, 講師 (50335270) ; SAKAMOTO Tatsunori 京都大学, 大学院医学研究科, 助教 (60425626) ; KITAJIRI Shinichiro 京都大学, 大学院医学研究科, 助教 (00532970) ; EBISU Fumi 京都大学, 大学院医学研究科, 特定研究員 (70596197)
• Co-Investigator(Renkei-kenkyūsha): FUNABIKI Kazuo (00301234)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 内耳有毛細胞 / 遺伝子導入 / コンフォーカルマイクロエンドスコープ

Outline of Final Research Achievements

At present, it is difficult to transfer genes into hair cells in inner ear. Also it is hard to transfer genes into only hair cells. In this study, we identified hair cell and tried to induce GFP into hair cells. Using glass micro-pipette, we injected GFP gene into inner ear tissues and passed an electric current. By adding electric current, the transduction efficiency increased. Furthermore, we attached glass pipette to cell surface and make micropore chemically and passed weak electric current. In this method, we could successfully induce GFP genes into hair cells. However, the efficiency was not good.
Also using confocal micro endoscope, we could observe hair cells.

Free Research Field

耳鼻咽喉科