2013 Fiscal Year Final Research Report
Functional analysis of the PI3K/Akt signaling pathway during the mammalian inner ear development and its application for regenerative medicine
| Project/Area Number |
23592496
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Juntendo University |
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Principal Investigator |
MURATA junko 順天堂大学, 医学部, 准教授 (80332740)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Thoru 北里大学, 理学部, 教授 (50280962)
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| Project Period (FY) |
2011 – 2013
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| Keywords | Pi3K/Aktシグナル伝達系 / PTEN / PTEN ckoマウス / ほ乳類 / 内耳発生 / 感覚上皮予定領域 / 有毛細胞 |
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| Research Abstract |
PTEN, a product of a tumor suppressor gene, inhibits the PI3K/Akt pathway by dephosphorylating phosphatidylinositol (3,4,5)-triphosphate. We tried to elucidate the functions of PTEN during mammalian cochlear development by deleting PTEN conditionally in the mouse inner ear.
In PTEN-conditional knockout (cko) mutant cochlea, the diameter of cochlear ducts was increased and the cell proliferation continued till the later stage outside of the prosensory region. Extra rows of OHCs and IHCs were observed, whereas the average length of the cochlear ducts was shorter than that of the control mice. The expression patterns of early prosensory markers were almost same between the PTEN-CKO and the control mice. However, the hair cell differentiation was accelerated in the PTEN-CKO cochleae. The results indicate that PTEN may regulate the cell differentiation and proliferation after the prosensory determination, and contribute to the convergent extension of the cochlear duct.
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Research Products
(17 results)
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[Presentation] Expression of TRPM4 in the mouse cochlea and its putative roles in the potassium ion transport and the inner hair cell repolarization2014
Author(s)
Murata J, Sakuraba M, Teruyama R, Kamiya K, Yamaguchi J, Okano H, Uchiyama Y, Ikeda K
Organizer
(The 37th Annual Midwinter Meeting of ARO
Place of Presentation
Manchester Grand Hyatt, San Diego, CA, USA
Year and Date
20140222-26
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