2013 Fiscal Year Final Research Report
Dendritic cell-targeted nasal vaccine development
| Project/Area Number |
23592513
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Masashi 大分大学, 医学部, 教授 (60211314)
NOMI Nozomi 大分大学, 医学部, 助教 (40468020)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 樹状細胞 / 上気道 / 粘膜免疫 / 経鼻ワクチン |
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| Research Abstract |
Dendritic cells (DCs) are essential for the induction of antigen (Ag)-specific immune responses, DC-targeted vaccination might be an effective strategy for the induction of the specific immune responses. A DNA plasmid encoding the P6 outer membrane protein of nontypeable Haemophilus influenzae (NTHi) was constructed. Mice were immunized 3 times intranasally with the DNA plasmid and Matrix-M, an immunostimulatory complex adjuvant. P6-specific nasal wash IgA and serum IgG were elevated following immunization with the DNA plasmid and Matrix-M. The number of specific IgA-producing cells increased in the nasal passages of the immunized mice. Moreover, DNA vaccination enhanced bacterial clearance. These findings suggest that a successful DNA vaccination protocol has been developed. Nasal vaccination with P6 DNA vaccine and Matrix-M might be a new effective regimen for the induction of specific protective immunity in the upper respiratory tract.
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[Journal Article]2011
Author(s)
Hirano T, Kodama S, Kawano T, Maeda K, Suzuki M. Monophosphoryl lipid A induced innate immune responses via TLR4 to enhance clearance of nontypeable Haemophilus influenzae and Moraxella catarrhalis fron the nasopharynx in mice.
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Journal Title
FEMS immunol Med Microbiol
Volume: 63
Pages: 407-417
Peer Reviewed
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