2013 Fiscal Year Final Research Report
Regulation of ultraviolet-induced actiation of p38, epithelial-mesenchymal transition, and apoptosis in corneal epithelial cells
| Project/Area Number |
23592591
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | National Defense Medical College |
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Principal Investigator |
KATO Naoko 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 病院, 講師 (20398605)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 角膜上皮細胞 / 紫外線 / 上皮間葉系移行 / アポトーシス / 酸化ストレス |
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| Research Abstract |
We irradiated ultraviolet onto the cultured corneal epithelial cells with various conditions. Phosphorylation of p38, decrease of E-cadherin, beta-catenin, and upregulation of a transcriptional factor, snail occurred, however, the representative mesenchymal markers were not induced.
We suspected that ultraviolet alone was insufficient for induction. We also assessed the addition of cytokines (TGF-beta and/or TNF-alpha), however the combined stimuli with ultraviolet and cytokines did not success induction of epithelial mesenchymal transition characterized by upregulation of mesenchymal markers.
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