2014 Fiscal Year Final Research Report
Establishment of serum and feeder-free cultured method in human corneal limbal epithelium
| Project/Area Number |
23592618
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOO Seiichi 東京大学, 医学部附属病院, 特任研究員 (20345052)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Keywords | 幹細胞疲弊症 / 無血清 / 無フィーダー |
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| Outline of Final Research Achievements |
Cultured human corneal epithelial sheet transplantation enable us to treat corneal stem cell deficiency, but this culture technique required bovine serum and mouse 3T3 feeder cells for successful culture. We established culture technique of human corneal limbal sheet with basal medium, B-27 supplement and KGF and are performing clinical study with this undifferentiated culture sheet.
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| Free Research Field |
眼科学
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