The development of 3D cartilage regeneration therapy using the human auricular cartilage progenitor cells with autologous serum.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592650
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Yokohama City University
• Principal Investigator: KOBAYASHI Shinji 横浜市立大学, 医学(系)研究科(研究院), 客員准教授 (90464536)
• Co-Investigator(Kenkyū-buntansha): MAEGAWA Jirou 横浜市立大学, 医学研究科, 教授 (70244449) ; TANIGUCHI Hideki 横浜市立大学, 医学研究科, 教授 (70292555)
• Project Period (FY): 2011 – 2013
• Keywords: 軟骨前駆細胞 / 軟骨再生 / 軟骨膜細胞 / 軟骨膜

Research Abstract

We have succeeded in isolating Human cartilage progenitor cells (hCPCs) and regenerating cartilage derived from them. The hCPCs which were isolated from auricular perichondrium had not only high proliferative and multipotent capacity that human chondrocytes (hCs) did not have, but had same cartilage extracellular matrix capacity as hCs.
Our purpose is to develop handing skill that could apply hCPCs in clinical setting on the basis of their culture technique. Specifically, there are (1) Implementation of hCPCs characterization (2) Evaluation of regenerated cartilage in vivo (3) The development of 3D cartilage regeneration derived from hCPCs (4) The development of autologous serum technique.

Research Products

• [Journal Article] Elastic cartilage reconstruction by transplantation of cultured hyaline cartilage-derived chondrocytes (2014)
  • Author(s): M Mizuno, T Takebe, S Kobayashi, Shintarou Kimura, M Masutani, S Lee, YH Jo, J Ik Lee, H Taniguchi
  • Journal Title: Transplant Proc Volume: 46(4) Pages: 1217-21
  • Peer Reviewed
• [Journal Article] Reconstruction of joint hyaline cartilage by autologous progenitor cells derived from ear elastic cartilage (2014)
  • Author(s): M Mizuno, S Kobayashi, T Takebe, H Kan, Y Yabuki, T Matsuzaki, H Y. Yoshikawa, S Nakabayashi, J,I Lee, J Maegawa, H Taniguchi
  • Journal Title: Stem Cells Volume: 32(3) Pages: 816-21
  • Peer Reviewed
• [Journal Article] Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor (2012)
  • Author(s): T Takebe, S Kobayashi H Kan, Hiromu Suzuki, M Mizuno, Y Yabuki, TAdegawa, T Yoshioka , J Tanaka, J Maegawa, H Taniguchi
  • Journal Title: Transplantation Proceedings Volume: 44(4) Pages: 1158-61
  • Peer Reviewed
• [Journal Article] Presence of cartilage stem/progenitor cells in adult mice auricular perichondrium (2011)
  • Author(s): S Kobayashi,, T Takebe, M Mizuno, J Maegawa, H Taniguchi
  • Journal Title: PLoS ONE Volume: 6(10) Pages: e26393
  • Peer Reviewed
• [Journal Article] Reconstruction of human elastic cartilage by a CD44^+CD90^+stem cell in the ear perichondrium (2011)
  • Author(s): S Kobayashi, T Takebe, M Inui, S Iwai, H Kan, Y Zheng, J Maegawa, H Taniguchi
  • Journal Title: Proc Natl Acad Sci USA Volume: 108(35) Pages: 14479-14484
  • Peer Reviewed
• [Journal Article] 軟骨再生医療:こどもの心をいやす医療に向けて最新のコラーゲンサイエンス-生物多様性が促進するバイオ新素材の変革-BIO INDUSTRY (2011)
  • Author(s): 小林眞司、谷口英樹
  • Journal Title: シーエムシー出版 Volume: BIO INDUSTRY Vol 18 No11 Pages: 32-37
• [Presentation] ヒト耳介軟骨幹/前駆細胞における自家血清培養法の検証 (2012)
  • Author(s): 矢吹雄一郎、小林眞司、水野満、廣冨浩一、安村和則、武部貴則、前川二郎、谷口英樹
  • Organizer: 第21回形成外科学会基礎学術集会
  • Place of Presentation: 福島
  • Year and Date: 20121004-05
• [Presentation] Reconstruction of human elastic cartilage by progenitor cells in the ear perichondrium (2012)
  • Author(s): Suzuki H, Kobayashi S, Takebe T, Mizuno M, Murata S, Yabuki Y, Hirotomi K, Yasumura K, Maegawa J, Taniguchi H
  • Organizer: International Society for Stem Cell Research 10^<th> annual meeting
  • Place of Presentation: Yokohama,Japan
  • Year and Date: 20120613-16
• [Presentation] Reconstitution of Articular(joint) cartilage defects by auricle(ear) derived stem/ progenitor cells (2012)
  • Author(s): Mizuno M, Kobayashi S Takebe T, Suzuki H, Murata S, Yabuki Y, Hirotomi K, Yasumura K, Maegawa J, Taniguchi H
  • Organizer: International Society for Stem Cell Research 10^<th> annual meeting
  • Place of Presentation: Yokohama,Japan
  • Year and Date: 20120613-16
• [Presentation] ヒト耳介軟骨膜由来幹/前駆細胞を用いた弾性軟骨再構築法の開発 (2012)
  • Author(s): 武部貴則、小林眞司、矢吹雄一郎、鈴木啓、水野満、安村和正、広富浩一、鄭允文、前川二郎、谷口英樹
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: 横浜
  • Year and Date: 20120112-14
• [Presentation] ヒト弾性軟骨膜細胞由来の再生軟骨を構成する軟骨膜の特性解析 (2011)
  • Author(s): 小林眞司、武部貴則、水野満、鈴木啓、矢吹雄一郎、鄭允文、前川二郎、谷口英樹
  • Organizer: 第20回日本形成外科学会基礎学術集会
  • Place of Presentation: 東京
  • Year and Date: 20111006-07
• [Presentation] Identification and utilization of human cartilage stem cells from the ear (2011)
  • Author(s): Suzuki H, Kobayashi S, Takebe T, Mizuno M, Yabuki Y, Yasumura K, Murata S, Maegawa J, Taniguchi H
  • Organizer: The 12th Congress of the Asian Society of Transplantation
  • Place of Presentation: Seoul, Korea
  • Year and Date: 20110925-28
• [Presentation] イヌ耳介由来軟骨幹/前駆細胞を用いた関節軟骨再生療法の開発 (2011)
  • Author(s): 水野満、小林眞司、武部貴則、鈴木啓、村田駿介、矢吹雄一郎、安村和則、前川二郎、谷口英樹
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20110919-21