2013 Fiscal Year Final Research Report
Effect of Adipose-Derived Stem Cells and Cross-linking of Collagen on Acellular Dermal Matrix
| Project/Area Number |
23592658
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 無細胞真皮 / 結合組織 / 再生医学 / 幹細胞 / 脂肪組織 |
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| Research Abstract |
It has been known that an acellular dermal matrix (ADM) is partially resorbed after transplanting. In this study, the effectiveness of adipose-derived stem cells (ASCs) and collagen cross-linking for the resorption of ADM was investigated. ADM was prepared from the skin of Fischer rats with trypsin. Cross-linking of ADM was performed with glutaraldehyde. ASCs from the inguinal fat pads were cultivated and the cells were seeded on ADMs. ADM with ASCs, cross-linked ADM and untreated ADM were respectively transplanted subcutaneously or into the defects of Achilles tendon of rats. As compared to the untreated ADMs, the subcutaneously transplanted ADM with ASCs significantly inhibited the ADM resorption. Although the cross-linked ADM inhibited the resorption, neo-vascularization was less induced than the ADM with ASCs. In the tendon regeneration, the ADMs with ASCs showed the better tendinous regeneration than the other types of ADMs. ASCs appeared effective for inhibiting ADM resorption.
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