Early diagnosis of the possibility of severe sepsis using NMR

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592668
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Kyoto University
• Principal Investigator: SUZUKI Takao 京都大学, 医学(系)研究科(研究院), 講師 (40328810)
• Co-Investigator(Kenkyū-buntansha): KOIKE Kaoru 京都大学, 大学院医学研究科, 教授 (10267164) ; HIRAKAWA Keiko 日本医科大学, 医学部, 助教 (30165162) ; SATO Norio 京都大学, 大学院医学研究科, 講師 (30409205)
• Project Period (FY): 2011 – 2013
• Keywords: 集中治療医学

Research Abstract

After extracting the removed brains and performing NMR measurement of the extracts, NMR data were analized. We found that 3 groups of L P S (10 mg / kg/day and 2 mg / kg) and saline treated were isolated in 6 hours after administration. No rats died within 6 hours. This finding suggests the possibility of identification of sepsis and its severity in the brain of early sepsis by this method. Although this finding is from ex vivo experiment, we consider it is important knowledge leading to early diagnosis of the possibility of severe sepsis with in vivo MRS sinse brain has less movement and it is very suitable for in vivo MRS measurement.