Development of intestinal adhesions prophylaxis after emergency surgery by IFN gamma- STAT1 -PAI-1 signal suppression

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592679
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: UEDA Kentaro 和歌山県立医科大学, 医学部, 助教 (20438279)
• Co-Investigator(Kenkyū-buntansha): KIDA Maki 和歌山県立医科大学, 医学部, 助教 (00326381) ; YONEMITSU Takafumi 和歌山県立医科大学, 医学部, 助教 (80382331)
• Project Period (FY): 2011 – 2013
• Keywords: マウス腸管癒着モデル / siPAI-1 HVJ vector / SOCS1 HVJ vector

Research Abstract

We constructed SOCS1 expression vector to suppress STAT1 specifically and PAI-1 siRNA vector, and examined the prevention effect of adhesion in mouse intestinal adhesion model using these vectors.
We had set the 5 group: A: Control group, B: SOCS1 group, C: PAI-1 siRNA group, D: SOCS1 + PAI-1 siRNA group, F: HGF (positive control). The results were equivalent to that of the F group was no difference in the three groups A, B, and C histology with the degree of adhesion, but adhesion is improved edge in the D group and E group. We found no side effects of bone marrow, liver, to the kidney in all groups.