2013 Fiscal Year Final Research Report
The functions of a new WD-repeat domain containing protein naofen to modulate LPS-induced liver dysfunction
| Project/Area Number |
23592689
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Aichi Medical University |
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Principal Investigator |
FENG Guo-Gang 愛知医科大学, 医学部, 講師 (70351111)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Takashi 名古屋大学, 医学(系)研究科(研究院), 特任助教 (10378052)
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| Project Period (FY) |
2011 – 2013
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| Keywords | エンドトキシン(LPS) / クッパー細胞ptosis / TNFα / caspase-3 / アポトーシス / Bcl-2 / Bcl-xL / cytochrome c |
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| Research Abstract |
Endothxin (LPS) causes hepatocytic apoptosis and liver dysfunction, as described in cases of sepsis. In this study, we therefore investigated the functions of a new WD-repeat domain containing protein naofen (WDR35) in LPS-induced liver dysfunction. LPS activated Kuppfer cells and released cytokines, such as TNF-alpha. Which then increased mRNA and protein expression of naofen in hepatocytes. Naofen/WDR35 inhibited expressions of anti-apoptotic proteins Bcl-2 and Bcl-xL, and stimulated apoptosis of hepatocytes. These results suggested that naofen may be involved in endotoxin-induced liver injury, and as a new therapeutic targets for endotoxin-induced liver injury therapy.
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