2013 Fiscal Year Final Research Report
myocardial interstitial serotonin and 5-hydroxyindole acetic acid levels during ischemia-reperfusion
| Project/Area Number |
23592691
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
AKIYAMA Tsuyoshi 独立行政法人国立循環器病研究センター, 研究所, 室長 (70202554)
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| Co-Investigator(Renkei-kenkyūsha) |
SONOBE Takashi 独立行政法人国立循環器病研究センター, 研究所, 流動研究員 (70548289)
SHIMIZU Shuji 独立行政法人国立循環器病研究センター, 研究所, 特任研究員 (80443498)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 心臓 / 虚血・再灌流 / セロトニン / 5-HIAA / マイクロダイアリシス法 |
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| Research Abstract |
Using microdialysis technique, we monitored myocardial interstitial 5-HT and its metabolite by monoamine oxidase (MAO), 5-HIAA levels in the ischemic region of anesthetized rat or rabbit during ischemia and reperfusion. In vehicle, 5-HT levels increased during ischemia, but 5-HIAA levels decreased during ischemia. After reperfusion 5-HT levels transiently increased and then declined. 5-HIAA levels increased after reperfusion and kept high level. Fluoxetine decreased 5-HT levels during ischemia, but did not change 5-HIAA levels after reperfusion. Pargyline increased 5-HT levels after reperfusion and decreased 5-HIAA levels after reperfusion. During ischemia, the carrier-mediated 5-HT efflux could contribute to the increase in 5-HT levels and the decrease in 5-HIAA levels could be ascribed to the reduction of MAO activity due to oxygen depletion and acidosis. After reperfusion, washout to blood as well as removal by uptake carrier could contribute to the decrease in 5-HT levels.
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[Journal Article] Medetomidine, anα_2-adrenergic agonist, activates cardiac vagal nerve through modulation of baroreflex control2012
Author(s)
Shimizu S, Akiyama T, Kawada T, Sata Y, Mizuno M, Kamiya A, Shishido T, Inagaki M, Shirai M, Sano S, Sugimachi M
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Journal Title
Circulation Journal
Volume: 76
Pages: 152-159
DOI
Peer Reviewed
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[Journal Article] Centrally administered ghrelin activates cardiac vagal nerve in anesthetized rabbits2011
Author(s)
Shimizu S, Akiyama T, Kawada T, Sonobe T, Kamiya A, Shishido T, Tokudome T, Hosoda H, Shirai M, Kangawa K, Sugimachi M
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Journal Title
Auton Neurosci
Volume: 162
Pages: 60-65
DOI
Peer Reviewed
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