Investigation of the role of MMP in bone healing with mineral and proteome analyses

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592694
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tohoku University
• Principal Investigator: SASANO YASUYUKI 東北大学, 歯学研究科(研究院), 教授 (30196191)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Megumi 東北大学, 大学院歯学研究科, 助教 (20431512)
• Co-Investigator(Renkei-kenkyūsha): TAKAHASHI Nobuhiro 東北大学, 大学院歯学研究科, 教授 (60183852)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 骨 / 修復 / 石灰化 / マイクロCT / 分析走査電子顕微鏡 / 頭蓋骨 / ラット / 発生・成長

Outline of Final Research Achievements

The present study was designed to investigate the process of calcification during bone healing in a standardized rat calvarial bone defect model. A standard defect with diameter 3.8 mm was made on the parietal bone of 12-week-old male Wistar rats under anesthesia. The healing bone was analyzed with micro-computed tomography and energy dispersive X-ray spectroscopy in weeks 1,2,4 and 8. The bone mineral density and the Ca/P ratio increased, whereas the C/Ca and C/P ratios decreased during bone healing. The present study demonstrated that the mineral components increase in density and mature in quality while organic components decrease. Our following study revealed the comparable calcification process during development of rat calvarial bone development.

Free Research Field

形態系基礎歯科学・口腔解剖学・組織学・発生学