2013 Fiscal Year Final Research Report
Molecular biological studies on the effects of fungi on invasion of host cells by periodontalpathogenic bacteria
| Project/Area Number |
23592709
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Ohu University |
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Principal Investigator |
TAMAI Riyoko 奥羽大学, 歯学部, 准教授 (90367566)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 歯周病原性細菌 / Porphyromonas gingivalis / 侵入 / Candida albicans / 真菌 / 混合感染 |
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| Research Abstract |
In this study, we investigated the effect of C. albicans on the adhesion and invasion of human gingival epithelial cells and gingival fibroblasts by Porphyromonas gingivalis. Heat-killed C. albicans and water-soluble mannoprotein-beta-glucan complex from C. albicans did not enhance P. gingivalis adhesion. However, pretreatment of the cells with heat-killed C. albicans or water-soluble mannoprotein-beta-glucan complex from C. albicans significantly enhanced P. gingivalis invasion. In addition, C. albicans and C. parapsilosis up-regulate galectin-3 secretion by human gingival epithelial cells and gingival fibroblasts. These results suggest that the dynamics of galectin-3 may participate in the increase in invasion of P. gingivalis which is gram negative bacteria because galectin-3 binds to LPS which exists in gram-negative bacteria outer membrane.
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