2013 Fiscal Year Final Research Report
Genome sequence analysis of biofilm-forming Streptococcus constellatus
| Project/Area Number |
23592724
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMANE Kazuyoshi 大阪歯科大学, 歯学部, 講師 (40388369)
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| Research Collaborator |
KAI P LEUNG U.S, Army Dental and Trauma Research Detachment, Institute of surgical research Director of Science
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| Project Period (FY) |
2011 – 2013
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| Keywords | バイオフィルム / ストレプトコッカス / ゲノム |
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| Research Abstract |
We isolated Streptococcus constellatus strain H39 from an apical abscess lesion. Scanning electron micrographs revealed that strain H39 had dense meshwork-like structures around the cells, which are a characteristic of exopolysaccharide-producing bacteria. Strain H39 grown in flow chambers formed biofilm. We reported a draft genome sequencing of strain H39 which can be used for further genetic study on biofilm formation mechanisms. The genome sequencing was performed by pyrosequencing. The putative gaps between contigs were closed by sequencing of polymerase chain reaction amplicons over the gaps. A high-quality draft genome sequence indicated that strain H39 had 3 ribosomal RNA genes, 45 transfer RNA genes and 1,926 putative protein-coding genes. We also found that strain H39 possessed genes encoding exopolysaccharide biosynthesis proteins and ABC-type polysaccharide transport proteins. These genes might contribute to the biofilm formation of S. constellatus.
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[Journal Article] Identification of the genes involved in the biofilm-like structures on Actinomyces oris K20, a clinical isolate from an apical lesion2013
Author(s)
Mashimo C, Kamitani M, Nambu T, Yamane K, Yamanaka T, Tatami T, Inoue J, Kamei M, Morita S, Fukushima H
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Journal Title
Jounal of Endodontics
Volume: 39(1)
Pages: 44-48
DOI
Peer Reviewed
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[Journal Article] Biofilm-forming capacity on clinically isolated Streptococcus constellatus from an odontogenic subperiosteal abscess lesion2013
Author(s)
Yamanaka T, Furukawa T, Yamane K, Nambu T, Mashimo C, Maruyama H, Inoue J, Kamei M, Yasuoka H, Horiike S, Leung KP, Fukushima H
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Journal Title
Journal of Bacteriology & Parasitology
DOI
Peer Reviewed
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