2013 Fiscal Year Final Research Report
Regulation of differentiation of hard-tissue-constituting cells by pH regulating proteins. Clarification of the mechanism and its application for regenerative medicine.
| Project/Area Number |
23592748
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATAGIRI Takenobu 埼玉医科大学, ゲノム医学研究センター病態生理部門, 教授 (80245802)
MIYAMOTO Yoichi 昭和大学, 歯学部口腔生化学講座, 准教授 (20295132)
YAMADA Atsushi 昭和大学, 歯学部口腔生化学講座, 講師 (50407558)
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| Project Period (FY) |
2011 – 2013
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| Keywords | pH / 骨芽細胞 / 軟骨細胞 / 炭酸脱水酵素 / モノカルボン酸輸送体 |
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| Research Abstract |
We found that carbonic anhydrase-9 (CA9) is expressed in mouse chondrocytes and monocarboxylate transporter-1 (MCT1) in osteoblasts, respectively. Hence we investigated the roles of CA9 and MCT1 in differentiation of chondrocytes and osteoblasts, respectively. CA9 was expressed in proliferating but not in hypertrophic chondrocytes in the growth plates of mice. Suppression of CA9 expression by its siRNA induced the hypertrophic differentiation in mouse primary chondrocytes, indicating that CA9 has a role to suppress hypertrophic differentiation of chondrocytes. Suppression of the expression of MCT1 in mouse osteoblasts induced lowered expression of alkaline phosphatase (ALP), while lactate, an MCT1 substrate, augmented the expression of ALP in osteoblasts. We also found that MCT1 positively regulates phosphorylation and nuclear translocation of Smad1/5/8 in osteoblasts after stimulation by bone morphogenetic protein-2.
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Research Products
(39 results)
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[Journal Article] Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes2013
Author(s)
Maruyama T, Miyamoto Y, Yamamoto G, Yamada A, Yoshimura K, Suzawa T, Takami M, Akiyama T, Hoshino M, Iwasa F, Ikumi N, Tachikawa T, Mishima K, Baba K, Kamijo R
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Journal Title
PLOS ONE
Volume: 8
Pages: e56984
DOI
Peer Reviewed
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[Journal Article] R848, a toll-like receptor 7 agonist, inhibits osteoclast differentiation but not survival or bone-resorbing function of mature osteoclasts2012
Author(s)
Miyamoto A, Takami M, Matsumoto A, Mochizuki A, Yamada T, Tachi K, Shibuya I, Nakamachi T, Shioda S, Baba K, Inoue T, Miyamoto Y, Yim M, Kamijo R
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Journal Title
Cytotechnology
Volume: 64
Pages: 331-339
DOI
Peer Reviewed
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[Journal Article] Cdc42 is required for chondrogenesis and interdigital programmed cell death during limb development2012
Author(s)
Aizawa R, Yamada A, Suzuki D, Iimura T, Kassai H, Harada T, Tsukasaki M, Yamamoto G, Tachikawa T, Nakao K, Yamamoto M, Yamaguchi A, Aiba A, Kamijo R
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Journal Title
Mechanisms of Development
Volume: 129
Pages: 38-50
DOI
Peer Reviewed
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[Journal Article] Shared and Distinct Functions of the Transcription Factors IRF4 and IRF8 in Myeloid Cell Development2011
Author(s)
Yamamoto M, Kato T, Hotta C, Nishiyama A, Kurotaki D, Yoshinari M, Takami M, Ichino M, Nakazawa M, Matsuyama T, Kamijo R, Kitagawa S, Ozato K, Tamura T
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Journal Title
DOI
Peer Reviewed
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[Journal Article] Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-α2011
Author(s)
Tsukasaki M, Yamada A, Suzuki D, Aizawa R, Miyazono A, Miyamoto Y, Suzawa T, Takami M, Yoshimura K, Morimura N, Yamamoto M, Kamijo R
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 410
Pages: 766-770
DOI
Peer Reviewed
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[Journal Article] Monocarboxylate transporter-1 is involved in activation of nuclear factorκB and expression of phagocyte-type NADPH-oxidase in mouse chondrocytic ATDC5 cells exposed to interleukin-1β2011
Author(s)
Yoshimura K, Miyamoto Y, Yasuhara R, Maruyama T, Akiyama T, Yamada A, Takami M, Suzawa T, Tunawaki S, Tachikawa T, Baba K, Kamijo R
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Journal Title
J. Biol. Chem
Volume: 286
Pages: 14744-14752
DOI
Peer Reviewed
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[Journal Article] Porphyromonas gingivalis-derived lysine gingipain enhances osteoclast differentiation induced by tumor necrosis factor-αand interleukin-1β, but suppresses that by interleukin-17A
Author(s)
Akiyama T, Miyamoto Y, Yoshimura K, Yamada A, Takami M, Hoshino M, Imamura T, Maruyama T, Suzawa T, Kohda T, Tanaka K, Baba K, Kamijo R
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Journal Title
J. Biol. Chem
Volume: (in press)
DOI
Peer Reviewed
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[Presentation] Monocarboxylate transporter-1は骨芽細胞におけるアルカリホスファターゼの発現に必要である2013
Author(s)
吉村健太郎,宮本洋一,星野真理江,宮本尚,斎藤章大,船登咲映,泉田恵理,槇宏太郎,山本松男,馬場一美,上條竜太郎
Organizer
日本口腔組織培養学会設立50周年記念学術大会
Place of Presentation
東京
Year and Date
20131123-24
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[Presentation] Cdc42 is crucial for facial and palatal development2013
Author(s)
Nakayama M, Yamada A, Aizawa R, Isobe T, Suzuki D, Suzuki W, Sato Y, Aiba A, Baba K, Mishima K, Yamamoto M, Maki K, Kamijo R
Organizer
2^<nd> Meeting of the International Association for Dental Research-Asia Pacific Region
Place of Presentation
Bangkok, Thailand
Year and Date
20130821-23
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[Presentation] 毛包内の神経堤由来細胞による象牙芽細胞分化誘導2012
Author(s)
森澤絵里,須澤徹夫,宮内知彦,高見正道,山田篤,宮本洋一,林竜平,西田幸二,大隅典子,馬場一美,上條竜太郎
Organizer
第11回日本再生医療学会総会
Place of Presentation
横浜
Year and Date
20120612-14
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[Presentation] Monocarboxylate transporter-1 is required for cell death in mouse chondrocyte-kike ATDC5 cells exposed to interleukin-1β; via late phase activation of NF-κB and expression of phagocyte-type NADPH-oxidase2011
Author(s)
Yoshimura K, Miyamoto Y, Yasuhara R, Maruyama T, Akiyama T, Yamada A, Takami M, Suzawa T, Tsunawaki S, Tachikawa T, Baba K, Kamijo R
Organizer
The Joint International Meeting Sponsored by The Jpn. Soc. for Interferon and Cytokine Research
Place of Presentation
Osaka
Year and Date
20110525-27
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[Book] The New Frontiers in Research for Oral Cancer2012
Author(s)
Takami M, Miyamoto A, Matsumoto A, Mochizuki A, Tachi K, Baba K, Inoue T, Mijung Y, Shibuya I, Zhao B, Kamijo R
Publisher
Maruzen Planet
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