2013 Fiscal Year Final Research Report
Isolation of thecandidate gene for the marker of the body time determinant in human saliva.
| Project/Area Number |
23592756
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
ONISHI Yoshiaki 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (60233219)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 口腔生化学 |
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| Research Abstract |
We have identified 22 genes which are controlled directly by clock genes and show circadian transcription by the combination of ChIP on Chip and microarray experiments using human salivary gland cell line HSG. Rev-erb alpha was included in the 22 genes as a positive control. Then, 9 genes including Rev-erb alpha were selected as genes expressing in the normal human salivary gland by RT-PCR. In these 9 genes we found that 3 genes except Rev-erb alpha showed clear circadian transcription. Finally we have succeeded to identify the gene whose product is detected in saliva by western blot analysis. These results suggest that the selected gene is the good candidate for the marker of the body time determinant in human saliva.
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