2013 Fiscal Year Final Research Report

Usefulness on detection of intracanal microbe with photodynamic agent and laser

Research Project

Project/Area Number	23592805
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Conservative dentistry
Research Institution	Ohu University
Principal Investigator	KIMURA Yuichi 奥羽大学, 歯学部, 教授 (60211877)
Co-Investigator(Kenkyū-buntansha)	IMAI Hiroaki (10265209)
Project Period (FY)	2011 – 2013
Keywords	光増感剤 / 根管治療 / ナノ粒子 / 細菌検査 / レーザー / 臨床診断 / 蛍光色素 / 歯内療法
Research Abstract	Methylene blue as a photosensitizer, and poly (lactic-co-glycolic acid) (PLGA) spherical nanoparticles loaded with chitosan (particle size 250 nm) were used to combine microbe in this study. DIAGNOdent laser device (wavelength 655 nm) was used to detect photosensitizer with nanoparticles combined to microbe, and this study was performed within the nanoparticles concentration range of 0.01, 0.1, 1, 10 mg/ml. Detection sensitivity was slight increased using methylene blue-coated PLGA nanoparticles, but some problems still remain to complete diagnosis.