2013 Fiscal Year Final Research Report
Study of inflammatory and anti-inflammatory mechanisms by endothelial cells in persistent periapical periodontitis
| Project/Area Number |
23592811
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 歯根肉芽種 / midkine / 誘導型一酸化窒素合成酵素 / CX3CL1 / 血管内皮細胞 / 遺伝子発現 / Porphyromonas gingivalis / LPS |
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| Research Abstract |
The aim of this study was to determine the function of midkine and iNOS inhibitor to endothelial cells in periapical granulomas. Midkine, iNOS and CXCL1 protein and mRNA expression in 69 periapical granulomas were detected, although healthy gingival tissues did not show those expressions. LPS was extracted from Porphyromonas gingivalis, which is known as potent periodontopathic bacteria. Then, human umbilical vein endothelial cells (HUVEC) were stimulated with the LPS. Stimulated HUVEC showed iNOS, midkine and CXCL1 protein and mRNA expression, but not after the application of iNOS inhibitor. The data are consistent with a hypothesis suggesting that endothelial cells in periapical granulomas express both iNOS and midkine, and these molecules may contribute tissue remodeling.
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