2013 Fiscal Year Final Research Report
Establishment of minimum invasive bone augmentation technique with nano-devices containing DNAs
| Project/Area Number |
23592864
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AKIRA Ishisaki (20356439)
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| Co-Investigator(Renkei-kenkyūsha) |
SHINJI Kuroda (50323689)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 歯科 / 骨組織再生 / 遺伝子導入 |
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| Research Abstract |
We have tried to establish a method of minimum invasive bone augmentation with nano-devices containing DNAs. Our preliminary experiment showed that direct in vivo gene transfer technique successfully introduced plasmid DNA of green fluorescence protein into cells of calvarial tissue. However, BMP-2 plasmid did not show positive effects for bone tissue regeneration in rat calvarial bone defect models. On the other hand, in another experiments to search the candidates of gene delivery, osteogenic differentiation of UE7T-13 cells, a bone marrow-derived hMSC line, was markedly enhanced by PDGF, although PDGF alone did not induce differentiation. These results suggest that the enhancement of TGF-beta(TGF)-inducedinduced osteogenic differentiation by PDGF-induced PI3K/Akt-mediated signaling depends on TGF-induced MEK activity. Thus, PDGF positively modulates the TGFinduced osteogenic differentiation of hMSCs through synergistic crosstalk between MEK- and PI3K/Akt-mediated signaling.
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