2013 Fiscal Year Final Research Report
The verification of the effects on the epidermal wound healing by the topical application of the mixture of cultured oral keratinocytes and hydrogel.
Project/Area Number |
23592882
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Dental engineering/Regenerative dentistry
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Research Institution | Niigata University |
Principal Investigator |
AJIMA hisao 新潟大学, 医歯学総合病院, 助教 (80377150)
|
Co-Investigator(Kenkyū-buntansha) |
MAEDA Takeyasu 新潟大学, 医歯学系, 教授 (40183941)
IZUMI Kenji 新潟大学, 医歯学系, 教授 (80242436)
|
Project Period (FY) |
2011 – 2013
|
Keywords | 口腔培養粘膜 / ハイドロミックスゲル / 創傷治療 |
Research Abstract |
Our long-term goal is to develop the current technology of oral mucosa keratinocyte culture system, and treat wounds by using cultured oral keratinocytes to regenerate epithelial defects. This study aimed to produce the mixture of cultured oral keratinocytes and hydrogel and assess the feasibility and effects on epidermal wound care such as burns and ischemic vicious ulcers. We measured the levels of various cytokines secreted from the mixture by ELISA assay. Based on measurement of the cytokine production, we found VEGF was sufficiently secreted into the culture supernatant although there were remarkable variations among samples. Furthermore, other cytokines also showed more remarkable variations in which some of them were undetectable. Thus, we concluded that these variations should have a negative impact on the in vivo animal study and it would be difficult to obtain the consistent outcomes. As a result, we may need to find a different strategy for cells to produce cytokines.
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