2013 Fiscal Year Final Research Report
Study of miRNA regulatory systems of antitumor effects by human cathelicidin.
| Project/Area Number |
23592938
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Michiyo 北海道大学, 理学部, 特任助教 (80316265)
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| Project Period (FY) |
2011 – 2013
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| Keywords | カセリシジン / 自然免疫 / 抗腫瘍効果 / 抗菌ペプチド / LL-37 / hCAP18 |
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| Research Abstract |
(1) hCAP18/LL-37 is the only antimicrobial peptide of the cathelichidins family identified from human. We reported Cathelicidin inhibits the enzumatic activity of MMP-9, thereby repressing tumor cell invasion and metastasis. In addition, miRNA microarrays were used to identify MMP-9-regulated miRNA, miR-4327. In conclusion, Cathelicidin regulates MMP-9 expression via miR-4327.
(2) We examined the effect of LL-37 peptide in angiogenesis. In angiostatic condition, LL-37 peptide inhibited the tube formation of HMVECs in dose dependently. By contrast, in proangiogenic condition, LL-37 peptide induced a 2.6-fold increase in tube formation compared with control. Additionally, miRNA microarrays were used to identify LL-37-regulated miRNA. In angiostatic condition, LL-37 could induce expression of miR-3188 and in proangiogenic condition, LL-37 could induce expression of miR-582-5p.In conclusion, LL-37 has angiostatic and proangiogenic activity in vascular endothelial cells.
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[Presentation] 624 Cathelicidin is a regulator of invasion in oral squamous-cell carcinoma2013
Author(s)
Okumura, K., Sawada, N., Isogai, E., Shibata, T. Isogai, H
Organizer
The 2nd Meeting of the International Association of Dental Research-Asia Pacific Region (IADR-APR)
Place of Presentation
Thailand, Bangkok,Plaza Athenee
Year and Date
20130821-23
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